| 摘要: |
| 目的：评价羚珠散治疗小鼠癫痫发作的预防作用及机制。方法：将25只C57BL/6小鼠随机分成3组，其中模型组和给药组每组各10只，正常组5只。采用腹腔注射戊四氮的方法制备癫痫模型，给药组每次造模前30 min灌胃羚珠散（剂量：1500 mg/kg）进行预防给药，每2 d重复一次造模和给药，共进行3次；正常组给予同体积0.9%氯化钠注射液。通过Racine评分评价造模后小鼠癫痫发作情况，于末次注射24h后分离各组小鼠大脑皮层和海马组织，通过Real-time PCR和免疫组化检测小鼠Gabbr1和Kcnt1基因蛋白表达情况。结果：Racine评分模型组显著高于正常组（P<0.0001），给药组显著低于模型组（P<0.0001）；HE染色切片显示，药物作用后可以有效减少海马体组织细胞的损伤，与正常组接近；给药组海马组织中Gabbr1基因表达显著高于模型组（P=0.0335），但与正常组比较，差异无统计学意义（P>0.05）；Kcnt1基因表达显著高于模型组（P=0.0139）与正常组（P=0.0063）。免疫组化结果显示，给药组中，小鼠海马组织Gabbr1和Kcnt1蛋白表达程度高于模型组（PGabbr1=0.0194，PKcnt1=0.0037）与正常组（PGabbr1=0.0094，PKcnt1=0.0255）。结论：羚珠散具有预防和抗小鼠癫痫的作用，其机制可能与钾离子通道KCNT1介导的抑制作用有关。 |
| 关键词: 癫痫 羚珠散 PTZ Gabbr1 Kcnt1 |
| DOI： |
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| Preventative effect of Lingzhu powder against seizure in mice and its mechanism |
| LU Weisheng,WANG Chengji,WANG Haijie |
| (Shanghai Laboratory Animal Research Center，Shanghai 201203，China) |
| Abstract: |
| Objective：To investigate the preventative effect of Lingzhu powder against seizure in mice and its mechanism.Methods：A total of 25 C57BL/6 mice were randomly divided into model group with 10 mice，administration group with 10 mice，and normal group with 5 mice.Intraperitoneal injection of pentetrazol was performed to establish a model of epilepsy；the mice in the administration group were given Lingzhu powder (at a dose of 1500 mg/kg) for prophylaxis by gavage at 30 minutes before modeling，and modeling and administration were performed once every 2 days for 3 times in total；the mice in the normal group were given an equal volume of 0.9% sodium chloride injection.Racine score was used to evaluate seizure after modeling；cerebral cortex and hippocampal tissue were isolated at 24 hours after the last injection，and real-time PCR and immunohistochemistry were used to measure the mRNA and protein expression levels of Gabbr1 and Kcnt1 in mice.Results：The model group had a significantly higher Racine score than the normal group (P<0.0001)，and the administration group had a significantly lower Racine score than the model group (P<0.0001).HE staining showed a significant reduction in cell injury in hippocampal tissue after administration，which was similar to the normal group.The administration group had a significantly higher mRNA expression level of Gabbr1 in hippocampal tissue than the model group (P=0.0335)，with no significant difference between the administration group and the normal group (P>0.05)，and the administration group had a significantly higher mRNA expression level of Kcnt1 than the model group and the normal group (P=0.0139 and 0.0063).Immunohistochemistry showed that compared with the model group and the normal group，the administration group had significantly higher protein expression levels of Gabbr1 and Kcnt1 in hippocampal tissue (Gabbr1：P=0.0194 and 0.0094；Kcnt1：P=0.0037 and 0.0255).Conclusion：Lingzhu powder can prevent and exert a therapeutic effect on epilepsy in mice possibly by the inhibitory role mediated by the potassium channel KCNT1. |
| Key words: epilepsy Lingzhu powder pentetrazol Gabbr1 Kcnt1 |
