| 摘要: |
| 目的：探讨健脾补肾法对结直肠癌裸鼠移植瘤模型Klotho及铜死亡相关蛋白FDX1及LIAS的影响。方法：将16只裸鼠皮下移植瘤模型分为对照组和中药组，每组各8只，对照组用0.4 ml0.9%氯化钠注射液灌胃，中药组用0.4 ml健脾补肾类中药颗粒灌胃。2组裸鼠培育14 d后，免疫组化检测肿瘤组织Klotho及铜死亡相关蛋白FDX1、LIAS的表达情况。结果：2组造模后致瘤率均达100%，中药组裸鼠精神及活跃程度较好；2组灌胃14 d后各死亡1只。2组裸鼠肿瘤的质量及体积比较，差异无统计学意义（P>0.05）。中药组裸鼠肿瘤组织中Klotho蛋白、FDX1蛋白阳性表达显著高于对照组（P<0.05）；2组裸鼠肿瘤组织中LIAS蛋白表达均呈阴性，差异无统计学意义（P>0.05）。结论：健脾补肾法可上调结直肠癌裸鼠移植瘤模型抑癌基因Klotho蛋白及铜死亡相关蛋白FDX1的表达。 |
| 关键词: 结直肠癌 健脾补肾法 Klotho 铜死亡相关蛋白 |
| DOI： |
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| Effect of spleen-strengthening and kidney-tonifying therapy on Klotho and cuproptosis-related proteins in a nude mouse xenograft tumor model of colorectal cancer |
| WANG Menghan,YUAN Wanjing,YAO Xin |
| (Hunan University of Chinese Medicine，Changsha 410208，Hunan，China;The Second Affiliated Hospital of Hunan University of Chinese Medicine，Changsha 410005，Hunan，China) |
| Abstract: |
| Objective：To investigate the effect of spleen-strengthening and kidney-tonifying therapy on Klotho and the cuproptosis-related proteins FDX1 and LIAS in a nude mouse xenograft tumor model of colorectal cancer.Methods：A total of 16 nude mice with subcutaneous xenograft tumor were divided into control group and traditional Chinese medicine (TCM) group，with 8 mice in each group.The mice in the control group were given 0.4 ml 0.9% sodium chloride injection by gavage，and those in the TCM group were given 0.4 ml spleen-strengthening and kidney-tonifying TCM drugs by gavage.After the nude mice were fed for 14 days，immunohistochemistry was used to measure the expression levels of Klotho and the cuproptosis-related proteins FDX1 and LIAS in tumor tissue.Results：Both groups achieved a tumorigenesis rate of 100% after modeling，and the nude mice in the TCM group had good levels of spirit and activity；1 nude mouse died in each group after 14 days of administration by gavage.There were no significant differences in tumor weight and volume between the two groups (P>0.05).Compared with the control group，the TCM group had significantly higher positive expression rates of Klotho and FDX1 proteins (P<0.05)，and both groups had negative expression of LIAS protein in tumor tissue，with no significant difference between the two groups (P>0.05).Conclusion：Spleen-strengthening and kidney-tonifying therapy can upregulate the expression of Klotho and the cuproptosis-related protein FDX1 in a nude mouse xenograft tumor model of colorectal cancer. |
| Key words: colorectal cancer spleen-strengthening and kidney-tonifying therapy Klotho cuproptosis-related protein |
