| 摘要: |
| 目的：建立山银花配方颗粒高效液相色谱（HPLC）特征指纹图谱。方法：采用Scienhome Column Kromasil C18色谱柱（4.6 mm×250mm，5μm），以乙腈-0.4%磷酸水溶液为流动相进行梯度程序洗脱（0～20min，3%→15%乙腈；20～45min，15%→24%乙腈；45～55min，24%→40%乙腈；55～65min，40%→3%乙腈；65～75min，3%乙腈），检测波长238nm，流速1.0ml/min，柱温30℃，进样量5μl，检测时间75min。结果：由10批实验室自制样品建立山银花配方颗粒HPLC特征指纹图谱，确定了8个共有峰，已解析出5个共有峰成分。结论：本方法灵敏度高、稳定性和重复性好，所建立的特征指纹图谱可用于山银花配方颗粒的鉴别和质量评价。 |
| 关键词: 山银花配方颗粒 高效液相色谱法 特征指纹图谱 |
| DOI： |
|
|
| Characteristic fingerprint of Lonicerae Flos formulated granules based on high-performance liquid chromatography |
| ZHOU Youyu,ZHOU Lili,QIAO Yong |
| (Hunan University of Chinese Medicine，Changsha 410208，Hunan，China) |
| Abstract: |
| Objective：To investigate the characteristic fingerprint of Lonicerae Flos formulated granules based on high-performance liquid chromatography (HPLC).Methods：HPLC was performed on a Scienhome Column Kromasil C18 column (4.6mm×250mm，5μm) with a mobile phase of acetonitrile-0.4% phosphoric acid for gradient elution (0～20min，3%→15% acetonitrile；20～45min，15%→24% acetonitrile；45～55min，24%→40% acetonitrile；55～65min，40%→3% acetonitrile；65～75min，3% acetonitrile)，at a flow rate of 1.0mL/min，a column temperature of 30℃，a sample size of 5μL，a detection wavelength of 238nm，and a detection time of 75 minutes.Results：Ten batches of samples made in the laboratory were used to establish the characteristic fingerprint of Lonicerae Flos formulated granules.Eight common peaks were identified，and five corresponding constituents were identified.Conclusion：This method has good sensitivity，stability，and reproducibility，and the characteristic fingerprint can be used for the identification and quality assessment of Lonicerae Flos formulated granules. |
| Key words: Lonicerae Flos formulated granules high-performance liquid chromatography characteristic fingerprint |
