| 摘要: |
| 目的：建立高效液相色谱（HPLC）法同时测定湘产枳壳中芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷和橙皮内酯的含量。方法：采用Venusil XBP C18（4.6mm×250mm，5μm）色谱柱，以乙腈-0.1%磷酸水为流动相（80〖DK〗∶20），流速1.0ml/min，柱温35℃，变波长检测。结果：芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷、橙皮内酯分别在100～2000ng、420～8400ng、32.12～642.4ng、260～5200ng、42.75～855ng内呈良好线性关系，平均回收率分别为98.7%、101.0%、100.7%、100.6%、98.6%，且精密度、重复性、稳定性良好；各批次样品中芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷、橙皮内酯的含量范围分别为0.48%～0.84%、4.44%～8.50%、0.19%～0.95%、3.03%～10.19%、0.16%～0.30%；聚类分析分为3类，即11号样品为一类，2号与10号样品为一类，其余样品为一类；橙皮苷含量在各批次样本中波动较大。结论：本研究建立了湘产枳壳中5种成分的HPLC含量测定方法，该方法简便，准确度高，可为湘产枳壳质量控制提供科学依据。 |
| 关键词: 湘产枳壳 HPLC法 含量测定 |
| DOI： |
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| Simultaneous content determination of five constituents in Fructus Aurantii produced in Hunan，China by high-performance liquid chromatography |
| LIU Shanshan,JIANG Bo,SHI Yuhe |
| (Hunan University of Chinese Medicine，Changsha 410208，Hunan，China;Hunan Center for Drug Evaluation，Certification and ADR Monitoring，Changsha 410013，Hunan，China) |
| Abstract: |
| Objective：To establish the high-performance liquid chromatography (HPLC) method for simultaneous content determination of narirutin，naringin，hesperidin，neohesperidin，and meranzin in Fructus Aurantii produced in Hunan，China.Methods：HPLC was performed on a Venusil XBP C18 column(4.6mm×250mm，5μm) with a mobile phase of acetonitrile-0.1% phosphoric acid (80∶20) at a flow rate of 1.0ml/min and a column temperature of 35℃，with multiple wavelengths.Results：Narirutin，naringin，hesperidin，neohesperidin，and meranzin showed a good linear relationship within the ranges of 100～2000ng，420～8400ng，32.12～642.4ng，260～5200ng，and 42.75～855ng，respectively (average recovery rate =98.7%，101.0%，100.7%，100.6%，and 98.6%)，with good precision，reproducibility，and stability.The ranges of the content of narirutin，naringin，hesperidin，neohesperidin，and meranzin were 0.48%～0.84%，4.44%～8.50%，0.19%～0.95%，3.03%～10.19%，and 0.16%～0.30%，respectively.The samples were classified into three categories based on the cluster analysis，with sample 11 in one category，samples 2 and 10 in one category，and the other samples in one category.The content of hesperidin fluctuated across samples.Conclusion：This study establishes the HPLC method for simultaneous content determination of five constituents in Fructus Aurantii produced in Hunan.This method is simple，convenient，and highly accurate and can provide a scientific basis for the quality control of Fructus Aurantii produced in Hunan. |
| Key words: Fructus Aurantii produced in Hunan high-performance liquid chromatography content determination |
