| 摘要: |
| 目的：建立护心康片多波长HPLC指纹图谱，并研究复方与组方药物指纹图谱峰的相关性。方法：采用C18色谱柱（4.6mm×200mm，5μm）；柱温35℃；0.1%磷酸溶液（A）-乙腈（B）为流动相梯度洗脱；流速1.0ml/min；检测波长分别为203nm（检测三七和黄芪）、270nm（检测丹参酮类）、286nm（检测丹参酚酸类和黄芪）。结果：建立了23个特征峰的护心康片HPLC指纹图谱，并对其特征峰进行了组方药物归属分析。结论：该方法能揭示复方主要色谱峰在组方药物中的归属，有效控制护心康片的质量。 |
| 关键词: 护心康片 多波长HPLC 指纹图谱 相关性分析 相似度评价 |
| DOI： |
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| A correlation analysis of chromatographic peaks of Huxinkang tablets and its components based on multi-wavelength high-performance liquid chromatography fingerprints |
| YUAN Qingzhao,ZHU Xiaojing,XU Qian |
| (The Affiliated Hospital of Hunan Academy of Chinese Medicine，Changsha 410006，Hunan，China;Inheritance Studio of 2013 Famous Old TCM Doctors and Experts，State Administration of Traditional Chinese Medicine，Changsha 410006，Hunan，China) |
| Abstract: |
| Objective：To establish the multi-wavelength high-performance liquid chromatography（HPLC）fingerprints of Huxinkang tablets，and to investigate the correlation between the chromatographic peaks of the compound prescription and its components.Methods：HPLC was performed on a C18 column（4.6mm×200mm，5μm）with a mobile phase of 0.1% phosphoric acid solution（A）-acetonitrile（B）for gradient elution，at a column temperature of 35℃，a flow rate of 1.0ml/min，and detection wavelengths of 203 nm for Panax notoginseng and Astragalus membranaceus，270 nm for tanshinone，and 286 nm for salvianolic acid and Astragalus membranaceus.Results：The HPLC fingerprints of Huxinkang tablets with 23 characteristic peaks were established，and these characteristic peaks were analyzed to determine their corresponding components.Conclusion：This method can determine major chromatographic peaks of the compound prescription and their corresponding components and thus effectively control the quality of Huxinkang tablets. |
| Key words: Huxinkang tablet multi-wavelength high-performance liquid chromatography fingerprint correlation analysis similarity evaluation |
