| 摘要: |
| 目的：探讨Th1（CD4+TNF-α+）/Th2（CD4+IL-4+）亚群漂移在大兔创伤性深静脉血栓（DVT）形成中的作用及消栓饮的治疗机制。方法：将45只新西兰健康大兔分为空白组、假手术组、0.9%氯化钠注射液组、低分子肝素钠组、消栓饮组，每组各9只，采用直接钳夹静脉+双后肢石膏固定的方法制备创伤性DVT模型后进行给药，于第1、3、7天完成给药4h后，采用流式细胞术检测各组大兔Th1（CD4+TNF-α+）、Th2（CD4+IL-4+）的含量变化及Th1（CD4+TNF-α+）/Th2（CD4+IL-4+）值。结果：给药第1、3、7天0.9%氯化钠注射液组、低分子肝素钠组、消栓饮组的Th1（CD4+TNF-α+）比例、Th2（CD4+IL-4+）比例及Th1（CD4+TNF-α+）/Th2（CD4+IL-4+）值与同时间节点空白组、假手术组比较，差异均有统计学意义（P<0.05）；给药第3、7天低分子肝素钠组、消栓饮组的Th1（CD4+TNF-α+）比例、Th2（CD4+IL-4+）比例及Th1（CD4+TNF-α+）/Th2（CD4+IL-4+）值与0.9%氯化钠注射液组比较，差异均有统计学意义（P<0.05。结论：DVT形成过程中存在Th1（CD4+TNF-α+）/Th2（CD4+IL-4+）亚群动态平衡被打破；中药消栓饮可以通过干预Th1（CD4+TNF-α+）、Th2（CD4+IL-4+）含量变化，维持两者的平衡来达到治疗目的。 |
| 关键词: 深静脉血栓 消栓饮 （CD4+TNF-α+）/Th2（CD4+IL-4+）亚群漂移 实验研究 |
| DOI： |
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| Effect of Xiaoshuan decoction on Th1（CD4+TNF-α+）/Th2（CD4+IL-4+）subset shift in rabbits with traumatic deep venous thrombosis |
| XU Wenjie,WANG Yong,PAN Deyin |
| (Hunan University of Chinese Medicine，Changsha 410208，Hunan，China;The Second Affiliated Hospital of Hunan University of Chinese Medicine，Changsha 410005，Hunan，China) |
| Abstract: |
| Objective：To investigate the role of Th1（CD4+TNF-α+）/Th2（CD4+IL-4+）subset shift in traumatic deep venous thrombosis（DVT）in rabbits and the therapeutic mechanism of Xiaoshuan decoction.Methods：A total of 45 healthy New Zealand rabbits were divided into blank group，sham-operation group，0.9% sodium chloride injection group，low-molecular-weight heparin sodium group，and Xiaoshuan decoction group，with 9 rabbits in each group.A rabbit model of traumatic DVT was established by direct vein clamping and plaster immobilization of both hind limbs，and then corresponding drugs were given.At 4 hours after administration on days 1，3，and 7，flow cytometry was used to measure the changes in the percentages of Th1（CD4+TNF-α+）and Th2（CD4+IL-4+），and Th1（CD4+TNF-α+）/Th2（CD4+IL-4+）ratio was calculated.Results：On days 1，3，and 7 of administration，the 0.9% sodium chloride injection group，the low-molecular-weight heparin sodium group，and the Xiaoshuan decoction group had significantly different percentages of Th1（CD4+TNF-α+）and Th2（CD4+IL-4+）and Th1（CD4+TNF-α+）/Th2（CD4+IL-4+）ratio compared with the blank group and the sham-operation group（P<0.05）.On days 3 and 7 of administration，there were significant differences in the percentages of Th1（CD4+TNF-α+）and Th2（CD4+IL-4+）and Th1（CD4+TNF-α+）/Th2（CD4+IL-4+）ratio between the low-molecular-weight heparin sodium group/Xiaoshuan decoction group and the 0.9% sodium chloride injection group（P<0.05）.Conclusion：Th1（CD4+TNF-α+）/Th2（CD4+IL-4+）balance is broken during DVT.The traditional Chinese medicine Xiaoshuan decoction can regulate the changes in the percentages of Th1（CD4+TNF-α+）and Th2（CD4+IL-4+）and thus maintain Th1（CD4+TNF-α+）/Th2（CD4+IL-4+）balance. |
| Key words: deep venous thrombosis Xiaoshuan decoction Th1（CD4+TNF-α+）/Th2（CD4+IL-4+）subset shift experimental study |
