| 摘要: |
| 目的：基于抗氧化活性评价优选禾本科芦笋多糖酶法提取工艺。方法：以活性部位总多糖浸出量与DPPH自由基清除率为评价指标进行评价，在单因素试验的基础上进行正交试验，优选禾本科芦笋多糖的提取工艺。结果：最佳酶法提取工艺为芦笋粉末加3.0%纤维素酶、pH 6.5的柠檬酸缓冲溶液，在45℃温浸提取1.5h，多糖提取量与DPPH自由基清除率分别达到66.58mg/g与65.24%。结论：正交试验法优选禾本科芦笋多糖的酶法提取工艺可行，该工艺为禾本科芦笋多糖的酶法提取优化提供了试验依据。 |
| 关键词: 禾本科芦笋 多糖 抗氧化 酶法提取 正交试验 |
| DOI： |
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| Optimization of enzymatic extraction process of polysaccharides from asparagus based on antioxidant activities |
| JIANG Bo,LIU Shanshan,WANG Lijun |
| (Hunan University of Chinese Medicine，Changsha 410208，Hunan，China;Hunan Center for Drug Evaluation and ADR Monitoring，Changsha 410013，Hunan，China) |
| Abstract: |
| Objective：To optimize the enzymatic extraction process of polysaccharides from asparagus based on antioxidant activities.Methods：With the amount of total polysaccharides from active parts and clearance rate of the free radicals of 2，2-diphenyl-1-picrylhydrazyl-hydrate（DPPH）as the assessment indices，an orthogonal test was performed based on single-factor experiment to optimize the extraction process of polysaccharides from asparagus.Results：The optimal enzymatic extraction process was asparagus powder with 3.0% cellulase and citric acid buffer（pH 6.5）for warm extraction at 45℃ for 1.5 hours.The extraction yield of polysaccharides reached 66.58mg/g，and the clearance rate of DPPH free radicals reached 65.24%.Conclusion：The enzymatic extraction process of polysaccharides from asparagus optimized based on the orthogonal test is feasible and provides an experimental basis for enzymatic extraction optimization for asparagus polysaccharides. |
| Key words: asparagus polysaccharides antioxidant enzymatic extraction orthogonal test |
