| 摘要: |
| 目的：建立紫玉簪Hosta ventricosa（ Salisb.） Stearn 的质量标准。方法：对紫玉簪的性状、显微特
征进行鉴别后，测定其水分、总灰分、酸不溶性灰分和浸出物含量，同时对指标成分吉托皂苷元和4-香豆酸进
行定性与定量分析，建立一套完整的紫玉簪质量标准体系。结果：紫玉簪性状和显微特征明显，薄层色谱斑点
清晰。吉托皂苷元在4.312 0～ 215.600 0 μg/mL（r=0.999 4）、4-香豆酸在1.009 0～ 50.448 2 μg/mL（r=1.000 0）
范围内呈现良好的线性关系；平均回收率分别为96.2%（RSD=3.21%）和94.5%（RSD=1.26%）。8 批次样品
水分、总灰分、酸不溶性灰分、浸出物、吉托皂苷元和4- 香豆酸含量范围分别为8.4% ～ 12.9%、4.3% ～ 5.6%、
0.4% ～ 1.8%、44.8% ～ 53.5%、0.133 ～ 0.955 mg/g 和0.063 ～ 0.154 mg/g。初步拟定水分、总灰分、酸不溶
性灰分限度分别不得超过13.0%、6.0% 和2.0% ；按干燥品计，浸出物不得少于40.0%，吉托皂苷元不得少于
0.013%，4-香豆酸不得少于0.008%。结论：本文建立了完整的性状鉴别、显微鉴别、薄层色谱鉴别、检查、浸出
物及含量测定方法，为合理评价紫玉簪药材质量提供了科学依据。 |
| 关键词: 紫玉簪 吉托皂苷元 4- 香豆酸 显微鉴别 薄层色谱法 高效液相色谱法 质量标准 |
| DOI： |
|
|
| Quality standard for Hosta ventricosa （Salisb.） Stearn |
| SHI Juan,ZHAO Qian,CHEN Gui,XU Zuogang,ZHANG Zhihong |
| (Qiannan Institute for Inspection and Testing, Duyun 558000, Guizhou, China;Qiannan People’s Hospital, Duyun 558000, Guizhou, China) |
| Abstract: |
| Objective: To establish the quality standard for Hosta ventricosa （Salisb.） Stearn. Methods: After
identifying the morphological characteristics and microscopic features of Hosta ventricosa, water content, total ash, acid-insoluble
ash, and extract were measured, and qualitative and quantitative analyses were performed for the marker components
gitogenin and 4-coumaric acid to establish a comprehensive quality standard system for Hosta ventricosa. Results: Hosta
ventricosa had distinct morphological and microscopic features, with clear spots on thin-layer chromatography （TLC）.
Gitogenin and 4-coumaric acid showed a good linear relationship within the range of 4.312 0-215.600 0 μg/mL （r =
0.999 4） and 1.009 0-50.448 2 μg/mL （r = 1.000 0）, respectively, with an average recovery rate of 96.2% （relative
standard deviation ［RSD］ = 3.21%） and 94.5% （RSD = 1.26%）, respectively. For the 8 batches of samples, the
ranges of water content, total ash, acid-insoluble ash, extract, gitogenin, and 4-coumaric acid were 8.4%-12.9%, 4.3%-
5.6%, 0.4%-1.8%, 44.8%-53.5%, 0.133-0.955 mg/g, and 0.063-0.154 mg/g, respectively. It was preliminarily proposed that water content, total ash, and acid-insoluble ash should not exceed 13.0%, 6.0%, and 2.0%, respectively; calculated
on the basis of the dried product, the extract should not be less than 40.0%, gitogenin should not be less than 0.013%, and
4-coumaric acid should not be less than 0.008%. Conclusion: This study established a comprehensive system for morphological
identification, microscopic identification, TLC identification, general examination, extract determination, and content
determination, which provides a scientific basis for rational evaluation of the quality of Hosta ventricosa. |
| Key words: Hosta ventricosa gitogenin 4-coumaric acid microscopic identification thin-layer chromatography high-performance liquid chromatography quality standard |
