| 摘要: |
| 目的：建立灵芝中23种成分含量的“一测多评”方法。方法：采用高效液相色谱法，色谱柱为 WATERS CORTECS C18（90 Å，2.7 µm，4.6 mm×150 mm）；流动相为乙腈- 0.075%（v/v）磷酸水溶液，梯度洗脱；检测波长252 nm；流速1.0 mL/min；柱温35℃；进样量10 µL。以灵芝酸 A 为内参物，计算其余22种成分（灵芝酸L、灵芝酸I、灵芝烯酸C、灵芝酸C2、赤芝酸C、赤芝酸N、灵芝酸C6、灵芝酸G、灵芝烯酸B、灵芝酸B、赤芝酸B、灵芝酸H、灵芝酸C1、赤芝酸D、灵芝酸F、灵芝酸J、灵芝马酮、灵芝酸DM、灵芝酸TR、灵芝醇F、灵芝醇B、灵芝醛A）的相对校正因子，并用其计算含量，与外标法测定结果进行比较。结果：23种成分在各自质量浓度范围内线性关系良好（R2>0.998），平均加样回收率为93.85%～110.91%。结论：所建立的以灵芝酸A为内参物的一测多评方法准确、重复性好，可用于灵芝中多指标成分的含量测定和质量控制。 |
| 关键词: 灵芝 灵芝三萜酸 高效液相色谱法 一测多评 相对校正因子 |
| DOI： |
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| A method of quantitative analysis of multi-components by single marker for 23 components in Ganoderma |
| WANG Yafeng,ZHU Lijun,CHEN Meihong,SHAO Shanshan,LIU Re,HU Lingjuan,WANG Xiaotong,XU Jing |
| (Zhejiang Shouxian Valley Pharmaceutical Co., Ltd., Jinhua 321200, Zhejiang, China;Jinhua Kangshou Pharmaceutical Co., Ltd., Jinhua 321200, Zhejiang, China;Zhejiang Provincial Key Laboratory of Biological Breeding and Comprehensive Utilization of Edible-Medicinal Fungi, Jinhua 321200, Zhejiang, China) |
| Abstract: |
| Objective: To establish a method of quantitative analysis of multi-components by single marker for 23 components in Ganoderma. Methods: High-performance liquid chromatography was performed on a WATERS CORTECS C18 column （90 Å, 2.7 μm, 4.6 mm × 150 mm） with a mobile phase of acetonitrile-0.075% （v/v） phosphoric acid solution for gradient elution at a wavelength of 252 nm, a flow rate of 1.0 mL/min, a column temperature of 35 ℃, and a sample size of 10 μL. With ganoderic acid A as the internal control, the relative correction factor was calculated for the other 22 components （ganoderic acid L, ganoderic acid I, ganoderenic acid C, ganoderic acid C2, lucidenic acid C, lucidenic acid N, ganoderic acid C6, ganoderic acid G, ganoderenic acid B, ganoderic acid B, lucidenic acid B, ganoderic acid H, ganoderic acid C1, lucidenic acid D, ganoderic acid F, ganoderic acid J, ganodermanontriol, ganoderic acid DM, ganoderic acid TR, ganoderiol F, ganoderiol B, and ganoderal A）, and their content was also calculated. These results were compared with the results of the external standard method. Results: All 23 components showed a good linear relationship within their respective range of mass concentration （R > 0.998）, with an average recovery of 93.85%-110.91%. Conclusion: The method of quantitative analysis of multi-components by single marker with ganoderic acid A as the internal control has good accuracy and reproducibility and can be used for the content determination and quality control of multi-target ingredients in Ganoderma. |
| Key words: Ganoderma ganoderma triterpene acid high-performance liquid chromatography quantitative analysis of multi-components by single marker relative correction factor |
