| 摘要: |
| 目的：观察雷火灸对脑缺血大鼠神经功能、相对脑缺血体积、缺血半暗带形态和神经生长因子（nerve growth factor，NGF）、原肌球蛋白受体激酶A（tropomyosin receptor kinase A，TrkA）表达的影响。方法：按随机数字表法将30只SD大鼠分为假手术组、模型组、雷火灸组，每组各10只；模型组、雷火灸组采用50%三氯化铁（ferric chloride，FeCl3）化学制备大脑中动脉血栓模型，雷火灸组给予艾灸“百会”干预，30 min/次，1次/d，连续干预7 d。采用Longa评分法评估各组大鼠神经功能，2，3，5-氯化三苯基四氮唑（2，3，5-triphenyltetrazolium chloride，TTC）染色法检测相对脑缺血体积，苏木精-伊红（hematoxylin and eosin，HE）染色法观察缺血半暗带组织形态变化，免疫组织化学法检测缺血半暗带NGF、TrkA的表达。结果：与假手术组比较，模型组大鼠Longa评分升高（P＜0.01），相对脑缺血体积增大（P＜0.01），缺血半暗带细胞构型紊乱，神经元大量变性坏死，NGF、TrkA表达增加（P＜0.05）。与模型组比较，雷火灸组大鼠Longa评分降低（P＜0.05），相对脑缺血体积缩小（P＜0.05），缺血半暗带神经元损伤程度减轻，NGF、TrkA表达增加（P＜0.05）。结论：雷火灸可能通过提高缺血半暗带NGF、TrkA的表达水平，改善脑缺血大鼠神经功能，缩小脑缺血体积，减轻脑损伤。 |
| 关键词: 脑缺血大鼠 雷火灸 神经功能 相对脑缺血体积 缺血半暗带 神经生长因子 原肌球蛋白受体激酶A |
| DOI： |
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| Effect of thunder-fire moxibustion on neurological function and the expression of nerve growth factor and tropomyosin receptor kinase A in the ischemic penumbra in a rat model of cerebral ischemia |
| YU Ruofan,YAO Tingting,ZHU Yongfa |
| (The Second Affiliated Hospital of Bengbu Medical University，Bengbu 233000，Anhui，China) |
| Abstract: |
| Objective：To investigate the effect of thunder-fire moxibustion on neurological function，relative cerebral ischemic volume，ischemic penumbra morphology，and the expression of nerve growth factor （NGF) and tropomyosin receptor kinase A （TrkA) in a rat model of cerebral ischemia.Methods：A total of 30 Sprague-Dawley rats were divided into sham-operation group，model group，and thunder-fire moxibustion group using a random number table，with 10 rats in each group.The rats in the model group and the thunder-fire moxibustion group were treated with 50% ferric chloride to establish a model of middle cerebral artery thrombosis，and those in the thunder-fire moxibustion group were given moxibustion at Baihui point for 30 minutes each time，once a day for 7 consecutive days.The Longa scoring method was used to assess neurological function；2，3，5-triphenyltetrazolium chloride staining was used to measure relative cerebral ischemic volume，and HE staining was used to observe histomorphological changes of ischemic penumbra；immunohistochemistry was used to measure the expression levels of NGF and TrkA in ischemic penumbra.Results：Compared with the sham-operation group，the model group had significant increases in Longa score （P < 0.01)，relative cerebral ischemic volume （P < 0.01)，and the expression levels of NGF and TrkA in ischemic penumbra （P < 0.05)，with disordered cell structure and massive degeneration and necrosis of neurons.Compared with the model group，the thunder-fire moxibustion group had significant reductions in Longa score （P < 0.05) and relative cerebral ischemic volume （P < 0.05)，as well as alleviation of neuronal injury and increases in the expression levels of NGF and TrkA in ischemic penumbra （P < 0.05).Conclusion：Thunder-fire moxibustion can improve neurological function，reduce cerebral ischemic volume，and alleviate brain injury in rats with cerebral ischemia by increasing the expression levels of NGF and TrkA in ischemic penumbra. |
| Key words: rats with cerebral ischemia thunder-fire moxibustion neurological function relative cerebral ischemic volume ischemic penumbra nerve growth factor tropomyosin receptor kinase A |
