| 摘要: |
| 目的：探讨复方藜芍片（Fufang Lishao tablets，FLS）对原发性三叉神经痛（primary trigeminal neuralgia，PTN）大鼠疼痛改善作用和对脑源性神经营养因子（brain-derived neurotrophic factor，BDNF）及其受体酪氨酸激酶受体B（tyrosine kinase receptor B，TrkB）表达的影响。方法：采用慢性压迫性损伤眶下神经术对60只雄性SD大鼠建立PTN模型，随机分为模型组、卡马西平组（20 mg/kg）、复方藜芍片低剂量组（FLS-L组，420 mg/kg）、复方藜芍片中剂量组（FLS-M组，840 mg/kg）、复方藜芍片高剂量组（FLS-H组，1 680 mg/kg），每组12只。对各组大鼠进行动物行为学评估，测定大鼠术侧面部机械痛敏阈值（mechanical withdrawal threshold，MWT）; ELISA法测定血清中白细胞介素- 6（interleukin-6，IL-6）、BDNF水平。Western blot测定三叉神经节（trigeminal ganglion，TG）中BDNF、TrkB表达水平。结果：与术前相比，手术后当天各组大鼠MWT值降低（P＜0.05），术后第28 d模型组MWT值为8.25，大鼠PTN模型造模成功;自术后第11天起，FLS-M、FLS-H组大鼠MWT值均高于与模型组（P＜0.05）;术后17～21 d，FLS-M、FLS-H组大鼠MWT值均高于卡马西平组（P＜0.05）。与模型组相比，FLS-M、FLS-H组大鼠血清IL-6水平下降（P＜0.05），复方藜芍片各剂量组大鼠血清BDNF及TG中BDNF、TrkB的表达水平均降低，差异均具有统计学意义（P＜0.05）。结论：复方藜芍片能够改善原发性三叉神经痛大鼠的痛觉过敏现象，可能与减轻炎症反应和抑制BDNF及其受体TrkB的表达有关。 |
| 关键词: 复方藜芍片 原发性三叉神经痛 白细胞介素-6 脑源性神经营养因子 酪氨酸激酶受体B |
| DOI： |
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| Effect of Fufang Lishao tablets on the expression of brain-derived neurotrophic factor and tyrosine kinase receptor B in rats with primary trigeminal neuralgia |
| LI Shunxin,SHI Weiling,GUO Shuyu |
| (Changsha Medical University，Changsha 410219，Hunan，China) |
| Abstract: |
| Objective：To investigate the effect of Fufang Lishao tablets (FLS) in improving pain in rats with primary trigeminal neuralgia (PTN)，as well as its effect on the expression of brain-derived neurotrophic factor (BDNF) and its receptor tyrosine kinase receptor B (TrkB).Methods：A total of 60 male Sprague-Dawley rats were selected，and the method of chronic constriction injury of the infraorbital nerve was used to establish a model of PTN.The rats were randomly divided into model group，carbamazepine (20 mg/kg) group，low-dose FLS group (FLS-L group，420 mg/kg)，middle-dose FLS group (FLS-M group，840 mg/kg)，and high-dose FLS group (FLS-H group，1 680 mg/kg)，with 12 rats in each group.Behavioral assessment was performed for the rats in each group，and mechanical withdrawal threshold (MWT) of face at the surgical side was measured;ELISA was used to measure the serum levels of interleukin-6 (IL-6) and BDNF;Western blot was used to measure the expression level of BDNF and TrkB in the trigeminal ganglion (TG).Results：After surgery，all groups had a significant reduction in MWT on the day of surgery (P＜0.05)，with an MWT value of 8.25 on day 28 after surgery，suggesting that the rat model of PTN was successfully established.Since day 11 after surgery，the FLS-M group and the FLS-H group had a significantly higher MWT value than the model group (P＜0.05)，and on days 17-21 after surgery，the FLS-M group and the FLS-H group had a significantly higher MWT value than the carbamazepine group (P＜0.05).Compared with the model group，the FLS-M group and the FLS-H group had a significant reduction in the serum level of IL-6 (P＜0.05)，and each FLS dose group had significant reductions in the serum level of BDNF and the expression levels of BDNF and TrkB in the TG (P＜0.05).Conclusion：FLS can improve hyperalgesia in rats with PTN，possibly by alleviating inflammatory response and inhibiting the expression of BDNF and its receptor TrkB. |
| Key words: Fufang Lishao tablets primary trigeminal neuralgia interleukin-6 brain-derived neurotrophic factor tyrosine kinase receptor B |
