| 摘要: |
| 目的：探究护心康含药血清通过调控核因子-E2-相关因子2-谷胱甘肽过氧化物酶4（Nrf2-GPX4）通路对抑制缺氧/复氧（H/R）大鼠H9C2心肌细胞铁死亡和细胞凋亡的影响。方法：构建H/R诱导的大鼠心肌细胞H9C2损伤模型，分为空白组、模型组、12%护心康含药血清组（12%护心康组）、6%护心康含药血清组（6%护心康组）及铁死亡抑制剂组（Fer-1组）。观察细胞凋亡率，丙二醛（MDA）、亚铁离子（Fe2+）、谷胱甘肽（GSH）、N-末端 B 型利钠肽原（NT-proBNP）水平，谷胱甘肽过氧化物酶4（GPX4）和核因子-E2-相关因子2（Nrf2）的表达。结果：模型组细胞凋亡率，MDA、Fe2+、GSH、NT-proBNP水平，Nrf2、GPX4蛋白表达水平，与空白组比较，差异均有统计学意义（P<0.05）；护心康各含药血清组及Fer-1 组细胞凋亡率及GSH、MDA、Nrf2蛋白表达水平，12%护心康组Fe2+、NT-proBNP水平，护心康各含药血清组GPX4蛋白表达水平，与模型组比较，差异均有统计学意义（P<0.05）。结论：护心康含药血清能减轻H/R诱导的H9C2心肌细胞铁死亡和凋亡，其机制可能与抑制Fe2+、MDA表达，上调Nrf2和GPX4蛋白表达有关。 |
| 关键词: 护心康 含药血清 缺氧/复氧损伤 H9C2心肌细胞 铁死亡 细胞凋亡 |
| DOI： |
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| Effect of Huxinkang on ferroptosis and apoptosis of H9C2 cardiomyocytes induced by hypoxia/reoxygenation |
| HE Xia,CHANG Jiayu,ZHU Xiaojing |
| (Hunan University of Chinese Medicine，Changsha 410208，Hunan，China;Hunan Provincial Hospital of Integrated Traditional Chinese and Western Medicine，Changsha 410006，Hunan，China) |
| Abstract: |
| Objective: To investigate the effect of serum containing Huxinkang in inhibiting the ferroptosis and apoptosis of H9C2 cardiomyocytes induced by hypoxia/reoxygenation (H/R) by regulating the nuclear factor erythroid 2-related factor 2 (Nrf2)-glutathione peroxidase 4 (GPX4) pathway.Methods: H9C2 rat cardiomyocytes were used to establish a model of H/R-induced injury and were then divided into blank group，model group，12% serum containing Huxinkang group (12% Huxinkang group)，6% serum containing Huxinkang group (6% Huxinkang group)，and ferroptosis inhibitor group (Fer-1 group).Cell apoptosis rate was observed，as well as the levels of malondialdehyde (MDA)，ferrous ion (Fe2+)，glutathione (GSH)，and N-terminal pro-brain natriuretic peptide (NT-proBNP) and the expression levels of GPX4 and Nrf2.Results: There were significant differences between the model group and the blank group in cell apoptosis rate，the levels of MDA，Fe2+，GSH，and NT-proBNP，and the protein expression levels of Nrf2 and GPX4 (P<0.05).There were significant differences between the Huxinkang groups/the Fer-1 group and the model group in cell apoptosis rate，the levels of GSH and MDA and the protein expression level of Nrf2; there were significant differences in the levels of Fe2+ and NT-proBNP between the 12% Huxinkang group and the model group; there was a significant difference in the protein expression level of GPX4 between the Huxinkang groups and the model group (P<0.05).Conclusion: Serum containing Huxinkang can alleviate ferroptosis and apoptosis of H9C2 cardiomyocytes induced by H/R，possibly by inhibiting the expression of Fe2+ and MDA and upregulating the protein expression of Nrf2 and GPX4. |
| Key words: Huxinkang serum containing hypoxia/reoxygenation injury H9C2 cardiomyocytes ferroptosis cell apoptosis |
