| 摘要: |
| 目的：建立毛茛中白头翁素的含量测定方法。方法：采用Merck Hibar C18色谱柱（4.6mm×250mm，5μm），流动相为甲醇-水（30∶70），检测波长220nm，流速0.8ml/min，柱温30℃，进样量20μL。理论板数按白头翁素计≥4000。结果：白头翁素在0.342～8.108mg/ml范围内线性关系良好，平均回收率为98.7%（n=9），相对标准偏差（RSD）为1.2%。结论：该方法简单、精密度高，结果准确可靠，可作为毛茛中白头翁素含量测定的一种有效方法。 |
| 关键词: 毛茛 白头翁素 高效液相色谱法 含量测定 |
| DOI： |
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| Content determination of anemonin in Ranunculus japonicus Thunb.by high-performance liquid chromatography：An experimental study |
| XIONG Jianbin |
| (Yueyang First People’s Hospital，Yueyang 414000，Hunan，China) |
| Abstract: |
| Objective：To establish a method for content determination of anemonin in Ranunculus japonicus Thunb.Methods：High-performance liquid chromatography was performed on a Merck Hibar C18 column (4.6mm×250mm，5μm) with a mobile phase of methanol-water (30∶70) at a detection wavelength of 220nm，a flow rate of 0.8ml/min，a column temperature of 30℃，and a sample size of 20μL.The number of theoretical plates for anemonin was ≥4000.Results：Anemonin showed a good linear relationship within the range of 0.342～8.108mg/ml，with an average recovery rate of 98.7% (n=9) (relative standard deviation=1.2%).Conclusion：This method is simple and accurate with reliable results and can be used as an effective method for the content determination of anemonin in Ranunculus japonicus Thunb. |
| Key words: Ranunculus japonicus Thunb anemonin high-performance liquid chromatography content determination |
