| 摘要: |
| 目的：探究心痛方对心肌梗死大鼠心肌组织内钠钙交换蛋白（NCX mRNA）的表达及钙离子（Ca2+）浓度的影响。方法：将50只SD大鼠分为假手术组、模型组、心痛方高剂量组、心痛方低剂量组、硫氮卓酮组，采用结扎大鼠冠状动脉左前降支的方法建立急性心肌梗死大鼠模型。运用RT-PCR法检测各组大鼠心肌细胞内NCX mRNA的表达，激光扫描共聚显微镜法检测大鼠心肌组织中的Ca2+浓度，HE染色法测定大鼠心肌梗死面积。结果：在下调NCX mRNA表达方面，心痛方高、低剂量组与假手术组、模型组、硫氮卓酮组比较，差异有统计学意义（P<0.01）；在降低MFI方面，心痛方高、低剂量组与假手术组、模型组比较，差异有统计学意义（P<0.05），且心痛方高剂量组在降低MFI方面还优于硫氮卓酮组（P<0.01）；在减少心肌梗死面积方面，心痛方高、低剂量组与模型组、硫氮卓酮组比较，差异有统计学意义（P<0.05）。结论：心痛方能下调急性心肌梗死大鼠NCX mRNA的表达，降低胞内Ca2+的浓度，从而抑制钙超载对心肌细胞的损害，减少心肌梗死面积。 |
| 关键词: 急性心肌梗死 心痛方 NCX mRNA Ca2+ 实验研究 |
| DOI： |
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| Effect of Xintong prescription on NCX mRNA expression and Ca2+concentration in rats with acute myocardial infarction |
| XIONG Zhiyu,FAN Jinru,CHEN Tong |
| (Hunan University of Chinese Medicine，Changsha 410208，Hunan，China;The First Affiliated Hospital of Hunan University of Chinese Medicine，Changsha 410007，Hunan，China) |
| Abstract: |
| Objective：To investigate the effect of Xintong prescription on NCX mRNA expression and calcium ion（Ca22+）concentration in myocardial tissue in rats with acute myocardial infarction.Methods：A total of 50 Sprague-Dawley rats were divided into sham-operation group，model group，high-and low-dose Xintong prescription groups，and diltiazem group，and ligation of the left anterior descending coronary artery was performed to establish a rat model of acute myocardial infarction.RT-PCR was used to measure the mRNA expression of NCX in cardiomyocytes，laser scanning confocal microscopy was used to measure the concentration of Ca22+ in myocardial tissue，and HE staining was used to measure myocardial infarct area.Results：The high-and low-dose Xintong prescription groups had a significantly greater reduction in the mRNA expression of NCX than the sham-operation group，the model group，and the diltiazem group（P<0.01）.The high-and low-dose Xintong prescription groups had a significantly greater reduction in mean fluorescence intensity than the sham-operation group and the model group（P<0.05），and the high-dose Xintong prescription group had a significantly greater reduction than the diltiazem group（P<0.01）.The high-and low-dose Xintong prescription groups had a significantly greater reduction in myocardial infarct area than the model group and the diltiazem group（P<0.05）.Conclusion：Xintong prescription can downregulate the mRNA expression of NCX，reduce the concentration of Ca22+，inhibit cardiomyocyte damage due to calcium overload，and thus reduce myocardial infarct area. |
| Key words: acute myocardial infarction Xintong prescription NCX mRNA Ca22+ experimental study |
