| 摘要: |
| 目的：探讨四逆软肝方对高浓度乙醛培养的人肝星状细胞肝纤四项（CG、HA、LN、PCIII）及基质金属蛋白酶1（MMP-1）、组织金属蛋白酶抑制剂1（TIMP-1）的影响。方法：采用高浓度乙醛对人肝星状细胞LX-2株造成损伤后，随机分为四逆软肝方、清热解毒化浊片、扶正化瘀片和脱离乙醛4组；经给药24h后搜集各组细胞培养液上清，采用ELISA试剂盒检测肝纤四项及MMP-1、TIMP-1含量。结果：250mmol/L乙醛培养72h，细胞存活率最高；与正常组比，乙醛组CG、HA、LN、MMP-1、TIMP-1差异无统计学意义（P>0.05），PCIII升高（P<0.01）。与正常组比较，脱离乙醛组CG下降（P<0.01）、PCIII升高（P<0.01）；与脱离乙醛组比较，四逆软肝方组HA、PCIII、TIMP-1均降低（P<0.01），CG、LN、MMP-1差异无统计学意义（P>0.05）。四逆软肝方组、清热解毒化浊片组、扶正化瘀片组组间比较，差异无统计学意义（P>0.05）。结论：四逆软肝方可降低受损的LX-2细胞HA、PCIII、TIMP-1指标的表达，可能对抗肝纤维化有一定的作用。 |
| 关键词: 四逆软肝方 高浓度乙醛 人肝星状细胞 肝纤四项 MMP-1 TIMP-1 实验研究 |
| DOI： |
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| Effect of Sini Ruangan prescription on four liver fibrosis markers，metal matrix proteinase-1，and tissue inhibitor of metalloproteinase-1 in human hepatic stellate cells cultured in high-concentration acetaldehyde |
| ZHAO Guo-rong,TANG Fei,MEI Ming |
| (Hunan University of Chinese Medicine，Changsha 410208，Hunan，China) |
| Abstract: |
| Objective：To investigate the effect of Sini Ruangan prescription on four liver fibrosis markers [cholyglycine（CG），hyaluronic acid（HA），laminin（LN），and procollagen III（PCIII）]，metal matrix proteinase-1（MMP-1），and tissue inhibitor of metalloproteinase-1（TIMP-1）in human hepatic stellate cells cultured in high-concentration acetaldehyde.Methods：After LX-2 human hepatic stellate cells were injured by a high concentration of acetaldehyde，these cells were randomly divided into Sini Ruangan prescription group，Qingre Jiedu Huazhuo tablets group，Fuzheng Huayu tablets group，and acetaldehyde-removed group.At 24 hours after administration，cell culture supernatants were collected from all groups，and ELISA was used to measure the levels of four liver fibrosis markers，MMP-1，and TIMP-1.Results：The cells cultured in 250 mmol/L acetaldehyde for 72 hours had the highest survival rate.There were no significant differences in the expression of CG，HA，LN，MMP-1，and TIMP-1 between the normal group and the acetaldehyde group（P>0.05），and compared with the normal group，the acetaldehyde group had a significant increase in PCIII（P<0.01）.Compared with the normal group，the acetaldehyde-removed group had a significant reduction in CG and a significant increase in PCIII（bothP<0.01）.Compared with the acetaldehyde-removed group，the Sini Ruangan prescription group had significant reductions in HA，PCIII，and TIMP-1（P<0.01），while there were no significant differences in CG，LN，and MMP-1 between the two groups（P>0.05）.There were no significant differences between the Sini Ruangan prescription group，the Qingre Jiedu Huazhuo tablets group，and the Fuzheng Huayu tablets group（P>0.05）.Conclusion：Sini Ruangan prescription can reduce the expression of HA，PCIII，and TIMP-1 in injured LX-2 cells and thus exerts an anti-liver fibrosis effect. |
| Key words: Sini Ruangan prescription high-concentration acetaldehyde human hepatic stellate cell four liver fibrosis markers metal matrix proteinase-1 tissue inhibitor of metalloproteinase-1 experimental study |
