| 摘要: |
| 目的：建立清咽利喉含片特征图谱，并测定芍药苷的含量。方法：采用Agilent Technologies 1 260Infinity Ⅱ 高效液相色谱仪，Ultimate XB-C18（4.6 mm×250 mm，5 μm）色谱柱，流速1.0 mL/min，以乙腈-1%冰乙酸溶为流动相进行梯度洗脱，柱温30℃，检测波长为254 nm，建立15批清咽利喉含片的高效液相特征图谱，并进行相似度评价;芍药苷的含量采用乙腈-0.1%磷酸溶液（14∶86），检测波长为230 nm。结果：建立了15批清咽利喉含片的高效液相特征图谱，标定了16个共有峰，共指认出5个成分（没食子酸、绿原酸、芍药苷、甘草苷、丹皮酚），15批样品相似度为0.519~0.998，相似度低于0.900的样品只有1批（S1）。芍药苷浓度在7.852 0～98.150 5 μg/mL范围内具有良好的线性关系（R2=0.999 5），平均加样回收率为101.97%，RSD为3.70%，芍药苷平均含量为3.52 mg/g。结论：本文建立的特征图谱及含量测定方法稳定、可行，可用于清咽利喉口含片及药材的质量评价。 |
| 关键词: 清咽利喉含片 芍药苷 特征图谱 质量评价 含量测定 |
| DOI： |
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| Characteristic chromatogram of Qingyan Lihou buccal tablets and content determination of paeoniflorin |
| CHEN Linglong,WANG Shuohui,LIANG Xuejuan |
| (Hunan Provincial Hospital of Integrated Traditional Chinese and Western Medicine，Changsha 410006，Hunan，China;Hunan Academy of Chinese Medicine，Changsha 410013，Hunan，China) |
| Abstract: |
| Objective: To establish the characteristic chromatogram of Qingyan Lihou buccal tablets，and to determine the content of paeoniflorin.Methods: The Agilent Technologies 1260 Infinity II high-performance liquid chromatography (HPLC） instrument was used to perform HPLC on an Ultimate XB-C18(4.6 mm × 250 mm，5 μm）column with acetonitrile-1% glacial acetic acid solution as the mobile phase for gradient elution at a flow rate of 1.0 mL/min，a column temperature of 30℃，and a detection wavelength of 254 nm.The HPLC characteristic chromatograms were established for 15 batches of Qingyan Lihou buccal tablets，and similarity evaluation was performed.Acetonitrile-0.1% phosphoric acid solution (14∶86） was used to determine the content of paeoniflorin at a detection wavelength of 230 nm.Results: The HPLC characteristic chromatograms were established for 15 batches of Qingyan Lihou buccal tablets，and 16 common peaks were identified，as well as 5 components of gallic acid，chlorogenic acid，paeoniflorin，liquiritin，and paeonol.The 15 batches of samples had a similarity of 0.519-0.998，with a similarity of <0.900 in only 1 batch (S1）.The concentration of paeoniflorin showed a good linear relationship within the range of 7.852 0-98.150 5 μg/mL (R2=0.999 5），with an average recovery of 101.97% and a relative standard deviation of 3.70%，and the mean content of paeoniflorin was 3.52 mg/g.Conclusion: The characteristic chromatogram and the method for content determination established in this study are stable and feasible and can be used for the quality assessment of Qingyan Lihou buccal tablets and medicinal materials. |
| Key words: Qingyan Lihou buccal tablets paeoniflorin characteristic chromatogram quality assessment content determination |
