| 摘要: |
| 目的：建立一种同时测定藏紫草中5种活性成分含量的方法。方法：采用Agilent SB-C18色谱柱（4.6 mm×250 mm，5 μm），以乙腈（A）-0.05%甲酸水（B）为流动相，检测波长510 nm，进样量为10 μL，流速为1 mL/min，柱温30 ℃;采用聚类分析、主成分分析对不同产地藏紫草进行质量评价。结果：不同产地藏紫草5种活性成分含量差异较大，左旋紫草素含量为0.002 7～0.041 4 mg/g，乙酰紫草素含量为0.015 8～1.157 0 mg/g，去氧紫草素含量为0.006 5～0.054 4 mg/g，异丁酰紫草素含量为0.047 7～0.549 9 mg/g，β-β’-二甲基丙稀酰阿卡宁含量为0.034 1～0.501 1 mg/g。聚类分析将15批藏紫草样品分为3类。通过主成分分析对藏紫草质量进行综合评价，西藏山南、林芝产地药材质量较佳。结论：本研究建立了同时测定藏紫草5种活性成分的HPLC方法，结合聚类分析、主成分分析可用于藏紫草药材的质量评价。 |
| 关键词: 藏紫草 质量评价 HPLC法 聚类分析 主成分分析 |
| DOI： |
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| Quantitative analysis of four active components in Radix Onosmatis and quality assessment based on high-performance liquid chromatography |
| XIE Jing,TANG Xueyang,ZHONG Can |
| (Institute of Traditional Chinese Medicine Resources，Hunan Academy of Chinese Medicine，Changsha 410013，Hunan，China) |
| Abstract: |
| Objective：To establish a method for simultaneous determination of five active components in Radix Onosmatis.Methods：High-performance liquid chromatography (HPLC) was performed on an Agilent SB-C18 column (4.6 mm × 250 mm，5 μm) with the mobile phase of acetonitrile (A)-0.05% formic acid solution (B) at a detection wavelength of 510 nm，injective volume at 10 μL，a flow rate of 1 mL/min，and a column temperature of 30 ℃.The cluster analysis and the principal component analysis were used to perform quality assessment of Radix Onosmatis from different producing areas.Results：There were significant differences in the content of five active components between Radix Onosmatis from different producing areas，with a content of 0.002 7-0.041 4 mg/g for shikonin，0.015 8-1.157 0 mg/g for acetylshikonin，0.006 5-0.054 4 mg/g for deoxyshikonin，0.047 7-0.549 9 mg/g for isobutyrylshikonin，and 0.034 1-0.501 1 mg/g for β-β’-dimethylacrylshikonin.The 15 batches of Radix Onosmatis were classified into three categories based on the cluster analysis.The principal component analysis was used for the comprehensive quality assessment of Radix Onosmatis，and the results showed that Radix Onosmatis from Shannan and Lingzhi of Tibet had a relatively good quality.Conclusion：This study establishes an HPLC method for simultaneous determination of five active components in Radix Onosmatis，and it can be used for the quality assessment of Radix Onosmatis in combination with the cluster analysis and the principal component analysis. |
| Key words: Radix Onosmatis quality assessment high-performance liquid chromatography cluster analysis principal component analysis |
