| 摘要: |
| 目的：探讨复方柴金解郁方对抑郁症合并失眠大鼠海马突触可塑性损伤的作用机制。方法：将72只实验大鼠随机分为空白组、慢性温和不可预知性应激(CUMS)组、睡眠剥夺（SD）组、CUMS+SD组、西药阳性药组及复方柴金解郁方2倍量（CJJY 2×）、1倍量（CJJY 1×）、0.5倍量（CJJY 0.5×）组，每组各9只。采用第1～35天CUMS联合第15～35天SD建立抑郁症失眠大鼠模型。通过行为学，外周及中枢氧化应激因子活性氧簇(ROS)、β位淀粉样前体蛋白裂解酶1(BACE1)和酪氨酸激酶受体4(ErBb4)，海马ErBb4、神经调节素1（NRG1）、N-甲基-D-天冬氨酸受体（NR）、α-突触核蛋白（SYN-α），海马神经元突触形态变化评估复方柴金解郁方的抗抑郁作用。结果：经药物干预后，西药阳性药组、CJJY-2×组血清与脑脊液ROS显著降低（P＜0.05，P＜0.01），海马ErBb4、NRG1、NR和SYN-α显著降低（P＜0.05，P＜0.01）；CJJY-1×、CJJY-0.5×组仅有脑脊液ROS显著降低（P＜0.05，P＜0.01）；西药阳性药组、CJJY-1×组血清与脑脊液BACE1均显著降低（P＜0.05，P＜0.01）；CJJY-1×组、CJJY-0.5×组仅有脑脊液BACE1显著降低（P＜0.05，P＜0.01）；提示CJJY 2×组和西药阳性药组改善海马神经元突触损伤效果最佳。结论：复方柴金解郁方可通过抑制BACE1调节NR/SNY-α信号改善抑郁症海马突触可塑性损伤。 |
| 关键词: 抑郁症 失眠 大鼠 复方柴金解郁方 海马 突触可塑性损伤 |
| DOI： |
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| Mechanism of action of compound Chaijin Jieyu prescription in improving hippocampal synaptic plasticity injury in rats with depression and insomnia |
| LI Zirong,LIU Deguo,XIE Sheng |
| (The First Affiliated Hospital of Guangxi University of Chinese Medicine，Nanning 530200，Guangxi，China) |
| Abstract: |
| Objective：To investigate the mechanism of action of compound Chaijin Jieyu prescription in improving hippocampal synaptic plasticity injury in rats with depression and insomnia.Methods：A total of 72 experimental rats were randomly divided into blank group，chronic unpredicted mild stress (CUMS) group，sleep deprivation (SD) group，CUMS+SD group，positive Western medicine group，2-fold compound Chaijin Jieyu prescription group (CJJY 2× group)，1-fold compound Chaijin Jieyu prescription group (CJJY 1× group)，and 0.5-fold compound Chaijin Jieyu prescription group (CJJY 0.5× group)，with 9 rats in each group.The method of CUMS on days 1-35 and CUMS+SD on days 15-35 was used to establish a rat model of depression and insomnia.The behavioral analysis was used to investigate the antidepressive effect of compound Chaijin Jieyu prescription，as well as the levels of ROS，BACE1，and ErBb4 in the peripheral and central nervous systems，the levels of ErBb4，NRG1，NR，and SYN-α in the hippocampus，and the morphological changes of hippocampal neuronal synapses.Results：After drug intervention，the positive Western medicine group and the CJJY-2× group had significant reductions in the level of ROS in serum and cerebrospinal fluid (P＜0.05，P＜0.01) and the levels of ErBb4，NRG1，NR，and SYN-α in the hippocampus (P＜0.05，P＜0.01)，while the CJJY-1× group and the CJJY-0.5× group only had a significant reduction in the level of ROS in cerebrospinal fluid (P＜0.05，P＜0.01).The positive Western medicine group and the CJJY-1× group had a significant reduction in the level of BACE1 in serum and cerebrospinal fluid (P＜0.05，P＜0.01)，and the CJJY-1× group and the CJJY-0.5× group only had a significant reduction in the level of BACE1 in cerebrospinal fluid (P＜0.05，P＜0.01)；These results suggested that the CJJY-2× group and the positive Western medicine group had the best improvement in hippocampal synaptic plasticity injury.Conclusion：Compound Chaijin Jieyu prescription can improve hippocampal synaptic plasticity injury in depression by inhibiting BACE1 and regulating the NR/SNY-α signal. |
| Key words: depression insomnia rat compound Chaijin Jieyu prescription hippocampus synaptic plasticity injury |
