| 摘要: |
| 目的：探讨黄芪皂苷与Notch信号通路蛋白表达的相关性，并进一步分析黄芪皂苷治疗骨质疏松症的作用机制。方法：选用3个月龄的雌性SD大鼠40只，采用随机数字表法将其随机分成假手术组、模型组、黄芪皂苷组、雌二醇组，每组各10只。采用摘除双侧卵巢的方法建立骨质疏松症的大鼠模型，造模后黄芪皂苷组予黄芪皂苷20 mg/（kg·d）灌胃，雌二醇组予戊酸雌二醇0.104 mg/（kg·d）灌胃，假手术组和模型组均予相同体积的0.9%氯化钠注射液灌胃。连续灌胃12周后取材收集血清和骨组织样本，观察各组大鼠股骨骨密度（BMD）、股骨矿物盐含量、股骨干骺端病理变化、血清中碱性磷酸酶（ALP）和抗酒石酸酸性磷酸酶（TRAP）含量、骨组织Notch2蛋白表达水平。结果：与假手术组相比，模型组大鼠的BMD、股骨矿物盐含量降低（P<0.05）；股骨干骺端松质骨小梁出现断裂和减少，排列变得稀疏，成骨细胞数量减少且结构模糊；血清中的ALP含量降低、TRAP含量升高（P<0.05），Notch2蛋白表达水平升高（P<0.05）。与模型组相比，黄芪皂苷组和雌二醇组大鼠的BMD、股骨矿物盐含量均升高（P<0.05）；股骨干骺端骨小梁排列更为致密，断裂现象减少，成骨细胞数量相对增多；血清中的ALP含量升高、TRAP含量降低（P<0.05）；Notch2蛋白表达水平降低（P<0.05）。结论：黄芪皂苷可能是通过Notch信号通路调控Notch2蛋白的表达来改善骨质疏松症。 |
| 关键词: 骨质疏松症 黄芪皂苷 Notch信号通路 SD大鼠 |
| DOI： |
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| Effect of astragaloside on osteoporosis in ovariectomized rats：A study based on the Notch signaling pathway |
| HUANG Jianying,XIAO Jingjing,LIU Xiaolan |
| (The Second Affiliated Hospital of Hunan University of Chinese Medicine，Changsha 410005，Hunan，China) |
| Abstract: |
| Objective：To investigate the association between astragaloside and the expression of proteins associated with the Notch signaling pathway，as well as the mechanism of action of astragaloside in the treatment of osteoporosis.Methods：A total of 40 female Sprague-Dawley rats，aged 3 months，were divided into sham-operation group，model group，astragaloside group，and estradiol group using a random number table，with 10 rats in each group.Both ovaries were removed to establish a rat model of osteoporosis，and after modeling，the rats in the astragaloside group were given astragaloside 20 mg/（kg·d） by gavage，those in the estradiol group were given estradiol valerate 0.104 mg/（kg·d） by gavage，while those in the sham-operation group and the model group were given an equal volume of 0.9% sodium chloride injection by gavage.After the drugs were given by gavage for 12 weeks，serum and bone tissue samples were collected from each group to observe the bone mineral density (BMD) of femur，the content of mineral salts in femur，the pathological changes of femur metaphysis，the content of alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP) in serum，and the protein expression level of Notch2 in bone tissue.Results：Compared with the sham-operation group，the model group had significant reductions in the BMD of femur and the content of mineral salts in femur (P<0.05)，with rupture，reduction，and loose arrangement of cancellous bone trabeculae in femur metaphysis，a reduction in the number of osteoblasts，and an unclear structure，as well as a significant reduction in ALP，a significant increase in TRAP，and a significant increase in the protein expression level of Notch2 (P<0.05).Compared with the model group，the astragaloside group and the estradiol group had significant increases in the BMD of femur and the content of mineral salts in femur (P<0.05)，with a dense arrangement of cancellous bone trabeculae，a reduction in rupture，and an increase in the number of osteoblasts，as well as a significant increase in ALP and significant reductions in TRAP and the protein expression level of Notch2 (P<0.05).Conclusion：Astragaloside can improve osteoporosis by regulating the expression of Notch2 via the Notch signaling pathway. |
| Key words: osteoporosis astragaloside Notch signaling pathway Sprague-Dawley rats |
