| 摘要: |
| 目的:基于网络药理学和实验验证探析“虎杖-威灵仙”药对治疗痛风性关节炎的作用机制。方法:通过TCMSP平台筛选“虎杖-威灵仙”药对的主要活性成分及作用靶点，应用GenneCards数据库预测痛风性关节炎的相关靶点，制作韦恩图获取“虎杖-威灵仙”药对与痛风性关节炎的共同靶点，运用Cytoscape 3.7.1软件绘制中药-成分-疾病-靶点调控网络，利用STRING数据库进行蛋白质-蛋白质相互作用网络的构建与分析。最后通过R语言和Bioconductor平台进行基因本体(GO)功能和京都基因与基因组百科全书（KEGG）通路富集分析，并通过动物实验进行验证。结果:“虎杖-威灵仙”药对主要通过12种活性成分、26个作用靶点及152个生物学通路发挥治疗痛风性关节炎的作用。其中主要核心靶点为白细胞介素-1B（IL-1B）、趋化因子配体2（CCL2）、基质金属蛋白酶9（MMP9）等；关键通路为白细胞介素-17（IL-17）信号通路。经过“虎杖-威灵仙”药对干预后，小鼠关节液中IL-17的含量有降低趋势，但差异无统计学意义（P>0.05）。结论:“虎杖-威灵仙”药对具有多成分、多靶点、多通路的特点，其作用机制可能与降低IL-17水平有关。 |
| 关键词: 痛风性关节炎 虎杖 威灵仙 作用机制 网络药理学 |
| DOI： |
|
|
| Mechanism of action of Polygonum cuspidatum-Clematis chinensis drug combination in treatment of gouty arthritis: A study based on network pharmacology and experimental validation |
| LI Yunhe,PANG Jing,LI Chunpu |
| (Tai’an Hospital of Traditional Chinese Medicine，Tai’an 271000，Shandong，China;Tai’an Central Hospital，Tai’an 271000，Shandong，China) |
| Abstract: |
| Objective: To investigate the mechanism of action of the Polygonum cuspidatum-Clematis chinensis drug combination in the treatment of gouty arthritis based on network pharmacology and experimental validation.Methods: The TCMSP platform was used to obtain the main active components and action targets of the Polygonum cuspidatum-Clematis chinensis drug combination，GenneCards database was used to predict the targets associated with gouty arthritis，and Venn diagram was plotted to obtain the intersecting targets between the Polygonum cuspidatum-Clematis chinensis drug combination and gouty arthritis.Cytoscape 3.7.1 was used to construct a traditional Chinese medicine-component-disease-target regulatory network，and STRING database was used to construct and analyze the protein-protein interaction network.R language and Bioconductor platform were used to perform gene ontology (GO) functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis，and animal experiments were conducted for validation.Results: The Polygonum cuspidatum-Clematis chinensis drug combination exerted a therapeutic effect on gouty arthritis through 12 active components，26 action targets，and 152 biological pathways.The main targets included IL-1B，CCL2，and MMP9，and the key pathway was the IL-17 signaling pathway.After intervention with the Polygonum cuspidatum-Clematis chinensis drug combination，there was a tendency of reduction in the content of IL-17 in synovial fluid (P>0.05).Conclusion: The Polygonum cuspidatum-Clematis chinensis drug combination exerts a therapeutic effect through multiple components，targets，and pathways，possibly by reducing the level of IL-17. |
| Key words: gouty arthritis Polygonum cuspidatum Clematis chinensis mechanism of action network pharmacology |
