| 摘要: |
| 目的：探讨在野百合碱（MCT）作用下三七总皂苷（PNS）对大鼠肺动脉平滑肌细胞（PASMCs）增殖、凋亡及其解整合素金属蛋白酶10/神经源性基因座notch同源蛋白-3(ADAM10/Notch3)通路的影响。方法：将体外培养的PASMCs随机分为正常组（C组）、野百合碱组（M组）和野百合碱+三七总皂苷组（P组），采用CCK-8法测各组细胞活力；EdU法检测细胞增殖；qPCR法和Western blot法分别检测细胞ADAM10、Notch3、人源重组蛋白1（Hes-1）、细胞周期蛋白依赖性激酶抑制因子（p27）、增殖细胞核抗原（PCNA）、半胱氨酸天冬氨酸蛋白酶3（Caspase-3）mRNA和蛋白的表达情况。结果：在MCT作用下，PASMCs细胞活力增强，EdU阳性率增加；ADAM10、Notch3、Hes-1、PCNA的mRNA以及蛋白表达上调（P<0.05，P<0.01)，p27和Caspase-3的mRNA以及蛋白表达下调(P<0.05，P<0.01）；使用PNS可下调MCT作用下的上述作用(P<0.05，P<0.01）。结论：三七总皂苷可通过抑制ADAM10/Notch3信号通路，减弱大鼠PASMCs增殖、促进PASMCs凋亡。 |
| 关键词: 肺动脉平滑肌细胞 三七总皂苷 野百合碱 ADAM10/Notch3通路 |
| DOI： |
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| Effect of Panax notoginseng saponins on the proliferation and apoptosis of pulmonary artery smooth muscle cells induced by monocrotaline and the ADAM10/Notch3 signaling pathway |
| HUANG Man,ZHANG Sai,TIAN Yunna |
| (Department of Pathophysiology，School of Basic Medical Sciences，Wenzhou Medical University，Wenzhou 325035，Zhejiang，China) |
| Abstract: |
| Objective：To investigate the effect of Panax notoginseng saponins (PNS) on the proliferation and apoptosis of rat pulmonary artery smooth muscle cells and the ADAM10/Notch3 signaling pathway under the action of monocrotaline (MCT).Methods：PASMCs cultured in vitro were randomly divided into normal group (group C)，MCT group (group M)，and MCT+PNS group (group P)，and CCK-8 assay was used to measure cell viability；the EdU method was used to measure cell proliferation；qPCR and Western blot were used to measure the mRNA and protein expression levels of ADAM10，Notch3，Hes-1，p27，proliferating cell nuclear antigen (PCNA)，and caspase-3.Results：Under the action of MCT，there were increases in the viability of PASMCs，EdU positive rate，and the mRNA and protein expression levels of ADAM10，Notch3，Hes-1，and PCNA(P<0.05，P<0.01），and there were reductions in the mRNA and protein expression levels of p27 and caspase-3(P<0.05，P<0.01），while PNS could reduce the above effects under the action of MCT(P<0.05，P<0.01）.Conclusion：PNS can weaken the proliferation of rat PASMCs and promote their apoptosis by inhibiting the ADAM10/Notch3 signaling pathway. |
| Key words: pulmonary artery smooth muscle cells Panax notoginseng saponins monocrotaline ADAM10/Notch3 signaling pathway |
