| 摘要: |
| 目的：观察六味地黄汤对腰椎融合术后新西兰兔模型骨融合的影响。方法：采用随机分组原则，将30只新西兰兔分为空白组、假手术组、对照组、阳性药物组、中药组，每组各6只，中药组、阳性药物组及对照组建立新西兰兔腰椎横突间融合模型，模型制备完成7 d后，中药组予六味地黄汤中药溶液10 mL/kg灌胃，阳性药物组予等量碳酸钙溶液灌胃，对照组、假手术组及空白组均予等量0.9%氯化钠注射液灌胃，每天2次，连续8周。给药结束后处死各组动物，检测其血清碱性磷酸酶（ALP）、骨形态蛋白2（BMP2）、Ⅰ型原胶原N端前肽（PINP）浓度值，并将融合骨块切片行HE染色观察。结果：药物干预后，中药组、阳性药物组血清ALP、BMP2、PINP水平均高于对照组、假手术组及空白组，差异有统计学意义（P＜0.05或P＜0.01），中药组血清各项指标水平高于阳性药物组，差异有统计学意义（P＜0.01）。HE染色结果显示，中药组融合骨块切片视野中植入骨与宿主骨的耦合、成骨细胞活跃程度均优于阳性药物组及对照组，炎性反应均较阳性药物组及对照组轻。结论：六味地黄汤可有效提升腰椎融合术后模型新西兰兔血清中ALP、BMP2、PINP等骨形成标志水平，促进腰椎融合术后植入骨与宿主骨的耦合，提高成骨细胞活跃度，减轻植骨融合处的炎性反应。 |
| 关键词: 腰椎融合术后 横突间融合 新西兰兔 六味地黄汤 实验研究 |
| DOI： |
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| Effect of Liuwei Dihuang decoction on bone fusion after lumbar fusion surgery in a rabbit model：An experimental study |
| PENG Yanyu,GAO Yong,ZHOU Yanlin |
| (Hunan University of Chinese Medicine，Changsha 410208，Hunan，China) |
| Abstract: |
| Objective：To investigate the effect of Liuwei Dihuang decoction on bone fusion after lumbar fusion surgery in a New Zealand rabbit model.Methods：A total of 30 New Zealand rabbits were randomly divided into blank group，sham-operation group，control group，positive drug group，and traditional Chinese medicine (TCM) group，with 6 rabbits in each group.The New Zealand rabbits in the TCM group，the positive drug group，and the control group were used to establish a model of lumbar intertransverse process fusion；after 7 days of modeling，the rabbits in the TCM group were given the solution of Liuwei Dihuang decoction at a dose of 10 mL/kg by gavage，those in the positive drug group were given an equal volume of calcium carbonate solution by gavage，and those in the control group and the sham-operation group were given an equal volume of 0.9% sodium chloride injection by gavage，twice a day for 8 consecutive weeks.The rabbits in all groups were sacrificed after the end of administration，and the serum concentrations of alkaline phosphatase (ALP)，bone morphogenetic protein 2 (BMP2)，and procollagen I N-terminal propeptide (PINP) were measured，while HE staining was performed for the sections of fused bone.Results：After drug intervention，the TCM group and the positive drug group had significantly higher serum levels of ALP，BMP2，and PINP than the control group，the sham-operation group，and the blank group (P＜0.05 or P＜0.01)，and the TCM group had significantly higher levels than the positive drug group (P＜0.01）.HE staining showed that compared with the positive drug group and the control group，the TCM group had better coupling between the bone graft and the host bone and a higher active degree of osteoblasts，with a slighter inflammatory response.Conclusion：In the New Zealand rabbit model after lumbar fusion surgery，Liuwei Dihuang decoction can effectively improve the levels of the bone formation markers ALP，BMP2，and PINP，promote the coupling between the implanted bone and the host bone，improve the active degree of osteoblasts，and alleviate inflammatory response at the site of bone graft fusion. |
| Key words: after lumbar fusion surgery intertransverse fusion New Zealand rabbit Liuwei Dihuang decoction experimental study |
