| 摘要: |
| 目的：基于网络药理学方法探讨茵陈干预肝纤维化的作用机制。方法：通过TCMSP数据库获取茵陈的活性成分及作用靶点；在GeneCards数据库和OMIM数据库中获取肝纤维化的相关靶点，将茵陈成分靶点与疾病靶点取交集，获得茵陈干预肝纤维化的作用靶点。运用Cytoscape 3.10.0构建化合物-靶点-疾病网络图，使用STRING数据库获得蛋白质-蛋白质相互作用(PPI)网络，通过Cytoscape 3.10.0进行可视化呈现及分析。使用Metascape数据库进行基因本体(GO)功能和京都基因与基因组百科全书（KEGG)通路富集分析。结果：筛选得到茵陈有效成分15个，作用靶点289个，其中肿瘤抑制蛋白基因53(TP53)、肿瘤坏死因子(TNF)、蛋白激酶1(AKT1)、白细胞介素-6(IL-6)、基质金属蛋白酶9(MMP9)、胱天蛋白酶3(CASP3)、缺氧诱导因子1A(HIF1A)和B细胞淋巴瘤2(BCL2)等为核心靶点。GO功能和KEGG通路富集分析结果主要涉及激素的反应、细胞迁移的正向调节、对细菌来源分子的反应、磷代谢过程的正向调节、对外源性刺激的反应等生物过程及癌症途径、血脂与动脉粥样硬化、磷脂酰肌醇-3-激酶-蛋白激酶(PI3K-Akt)、Janus激酶-信号传导及转录激活蛋白(JAK-STAT)、核因子-κB(NF-κB)等信号通路。结论：茵陈干预肝纤维化具有多成分、多靶点、多途径的特点，可为进一步研究提供参考。 |
| 关键词: 肝纤维化 茵陈 网络药理学 作用机制 |
| DOI： |
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| Mechanism of action of Artemisia capillaris in intervention of liver fibrosis：A study based on network pharmacology |
| LI Yongzhi,ZHENG Yu,LIU Senhao |
| (The Third Affiliated Hospital of Yunnan University of Chinese Medicine，Kunming 650011，Yunnan，China) |
| Abstract: |
| Objective：To investigate the mechanism of action of Artemisia capillaris in the intervention of liver fibrosis based on network pharmacology.Methods：TCMSP database was used to obtain the active components and action targets of Artemisia capillaris，and GeneCards and OMIM databases were used to obtain the targets associated with liver fibrosis.The action targets of the active components of Artemisia capillaris were intersected with the disease targets to obtain the action targets of Artemisia capillaris in the intervention of liver fibrosis.Cytoscape 3.10.0 was used to construct a component-target-disease network，STRING database was used to construct a protein-protein interaction network，and Cytoscape 3.10.0 was used for visualized representation and analysis.Metascape database was used to perform the gene ontology (GO) functional enrichment analysis and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis.Results：A total of 15 effective constituents were obtained for Artemisia capillaris，involving 289 action targets，among which the core targets included tumor protein 53，tumor necrosis factor，AKT1，interleukin-6，matrix metalloproteinase-9，caspase-3，hypoxia-inducible factor-1，and BCL2.The GO functional enrichment analysis and the KEGG pathway enrichment analysis showed that the main biological processes involved were response to hormone，positive regulation of cell migration，response to bacteria-derived molecules，positive regulation of phosphorus metabolic process，and response to exogenous stimulation，and the main signaling pathways involved were the cancer pathway，the lipid and atherosclerosis pathway，the PI3K-Akt pathway，the JAK-STAT pathway，and the NF-κB pathway.Conclusion：Artemisia capillaris has the features of multiple components，targets，and pathways in the intervention of liver fibrosis，which provides a reference for further research. |
| Key words: liver fibrosis Artemisia capillaris network pharmacology mechanism of action |
