| 摘要: |
| 目的：基于肠道菌群探讨参竹心康方对慢性心力衰竭（CHF）模型大鼠的治疗作用。方法：将50只大鼠随机分为空白组，模型组，参竹心康方低、中、高剂量组（简称为低、中、高剂量组），每组各10只。对模型组及各治疗组选择腹主动脉缩窄法制备CHF大鼠模型，最后模型组死亡3只，中、高剂量组各死亡1只，低剂量组死亡2只，其余造模成功。各治疗组予不同剂量参竹心康方灌胃，其余2组予0.9%氯化钠注射液灌胃，连续4周。测定血浆乳酸、脑钠尿肽（BNP）含量以及结肠内容物的菌群特征。结果：模型组乳酸、BNP含量，厚壁菌门丰度，拟杆菌门丰度，F/B值，Muribaculaceae菌属、Eubacterium菌属、Clostridia-UCG-014菌属、Prevotella菌属与空白组比较，差异均有统计学意义（P<0.05或P<0.01）。参竹心康方中剂量组乳酸含量及Eubacterium菌属、Prevotella菌属，低、中、高剂量组BNP含量及拟杆菌门丰度、Clostridia-UCG-014菌属，低、中剂量组厚壁菌门丰度、F/B值、Muribaculaceae菌属，与模型组比较，差异均有统计学意义（P<0.05或P<0.01）。结论：参竹心康方能明显改善CHF大鼠的心功能，其作用机制可能与改善肠道菌群紊乱及调节肠道菌群产物有关。 |
| 关键词: 慢性心力衰竭 肠道菌群 参竹心康方 实验研究 |
| DOI： |
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| Mechanism of action of Shenzhu Xinkang prescription in treatment of rats with chronic heart failure：A study based on intestinal flora |
| ZHAO Mengxue,ZHAO Qi |
| (Hunan University of Chinese Medicine，Changsha 410208，Hunan，China;Hunan Provincial Hospital of Integrated Traditional Chinese and Western Medicine，Changsha 410006，Hunan，China) |
| Abstract: |
| Objective：To investigate the therapeutic effect of Shenzhu Xinkang prescription on a rat model of chronic heart failure (CHF) based on intestinal flora.Methods：A total of 50 rats were randomly divided into blank group，model group，and low-，middle-，and high-dose Shenzhu Xinkang prescription groups (hereinafter referred to as low-，middle-，and high-dose groups)，with 10 rats in each group.For the rats in the model group and each treatment group，the abdominal aorta constriction method was used to establish a rat model of CHF，and successful modeling was achieved in all rats except 3 in the model group，1 each in the middle- and high-dose groups，and 2 in the low-dose group due to death.The rats in the treatment groups were given different doses of Shenzhu Xinkang prescription by gavage，and those in the other two groups were given 0.9% sodium chloride injection by gavage，for 4 consecutive weeks.Plasma lactic acid was measured，as well as the content of brain natriuretic peptide (BNP) and the characteristics of flora in colon contents.Results：There were significant differences between the model group and the blank group in lactic acid，BNP content，the abundance of Firmicutes and Bacteroidetes，F/B ratio，and the bacterial genera of Muribaculaceae，Eubacterium，Clostridia-UCG-014，and Prevotella (P<0.05 or P<0.01).There were significant differences in the content of lactic acid and the bacterial genera of Eubacterium and Prevotella between the middle-dose group and the model group；there were significant differences in the content of BNP，the abundance of Bacteroidetes，and the bacterial genus of Clostridia-UCG-014 between the low-，middle-，and high-dose groups and the model group；there were significant differences in the abundance of Firmicutes，F/B ratio，and the bacterial genus of Muribaculaceae between the low- and middle-dose groups and the model group (P<0.05 or P<0.01).Conclusion：Shenzhu Xinkang prescription can significantly improve the cardiac function of rats with CHF，possibly by improving intestinal flora disturbance and regulating intestinal flora products.Objective：To investigate the therapeutic effect of Shenzhu Xinkang prescription on a rat model of chronic heart failure (CHF) based on intestinal flora.Methods：A total of 50 rats were randomly divided into blank group，model group，and low-，middle-，and high-dose Shenzhu Xinkang prescription groups (hereinafter referred to as low-，middle-，and high-dose groups)，with 10 rats in each group.For the rats in the model group and each treatment group，the abdominal aorta constriction method was used to establish a rat model of CHF，and successful modeling was achieved in all rats except 3 in the model group，1 each in the middle- and high-dose groups，and 2 in the low-dose group due to death.The rats in the treatment groups were given different doses of Shenzhu Xinkang prescription by gavage，and those in the other two groups were given 0.9% sodium chloride injection by gavage，for 4 consecutive weeks.Plasma lactic acid was measured，as well as the content of brain natriuretic peptide (BNP) and the characteristics of flora in colon contents.Results：There were significant differences between the model group and the blank group in lactic acid，BNP content，the abundance of Firmicutes and Bacteroidetes，F/B ratio，and the bacterial genera of Muribaculaceae，Eubacterium，Clostridia-UCG-014，and Prevotella (P<0.05 or P<0.01).There were significant differences in the content of lactic acid and the bacterial genera of Eubacterium and Prevotella between the middle-dose group and the model group；there were significant differences in the content of BNP，the abundance of Bacteroidetes，and the bacterial genus of Clostridia-UCG-014 between the low-，middle-，and high-dose groups and the model group；there were significant differences in the abundance of Firmicutes，F/B ratio，and the bacterial genus of Muribaculaceae between the low- and middle-dose groups and the model group (P<0.05 or P<0.01).Conclusion：Shenzhu Xinkang prescription can significantly improve the cardiac function of rats with CHF，possibly by improving intestinal flora disturbance and regulating intestinal flora products. |
| Key words: chronic heart failure intestinal flora Shenzhu Xinkang prescription experimental study |
