| 摘要: |
| 目的：探讨象皮生肌膏对压疮模型大鼠创面愈合的影响及其作用机制。方法：将72只大鼠随机分为空白组、模型组、湿润烧伤膏组、象皮生肌膏组，每组各18只。空白组不予造模，其余3组建立Ⅲ期、Ⅳ期压疮模型。造模成功后空白组与模型组给予0.9%氯化钠注射液冲洗，湿润烧伤膏组给予湿润烧伤膏外用，象皮生肌膏组给予象皮生肌膏外用。干预14 d。于干预第3、7、14天观察创面愈合率，组织病理情况，白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）、血管内皮生长因子（VEGF）水平，p38丝裂原激活的蛋白激酶（p38MAPK）、MAPK激活蛋白激酶2（MK2）蛋白表达情况。结果：与模型组比较，湿润烧伤膏组、象皮生肌膏组治疗第3、7、14天创面愈合率均升高（P<0.05）。组织病理结果显示，治疗第14天，模型组可见少量毛细血管，湿润烧伤膏组、象皮生肌膏组可见大量成纤维细胞，毛细血管丰富。湿润烧伤膏组、象皮生肌膏组治疗第3、7、14天IL-6、TNF-α、VEGF、p38MAPK蛋白、MK2蛋白含量与模型组比较，差异均有统计学意义(P<0.05)。象皮生肌膏组治疗第7、14天IL-6含量及第7天TNF-α、VEGF含量与湿润烧伤膏组比较，差异均有统计学意义(P<0.05)。结论：象皮生肌膏能促进压疮创面修复，其作用机制可能与抑制p38MAPK、MK2蛋白表达，减少IL-6、TNF-α释放、调控VEGF合成有关。 |
| 关键词: 压疮 象皮生肌膏 大鼠 IL-6 TNF-α VEGF p38MAPK MK2 |
| DOI： |
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| Effect of Xiangpi Shengji ointment on wound healing in a rat model of pressure ulcers and its mechanism of action |
| PENG Lu,LIAO Ruoyi,TANG Yan |
| (Hunan University of Chinese Medicine，Changsha 410208，Hunan，China;The First Affiliated Hospital of Hunan University of Chinese Medicine，Changsha 410007，Hunan，China;The Second Affiliated Hospital of Hunan University of Chinese Medicine，Changsha 410005，Hunan，China) |
| Abstract: |
| Objective：To investigate the effect of Xiangpi Shengji ointment on wound healing in a rat model of pressure ulcers and its mechanism of action.Methods：A total of 72 rats were randomly divided into blank group，model group，Shirun Shaoshang ointment group，and Xiangpi Shengji ointment group，with 18 rats in each group.The rats in the blank group were not used for modeling，and those in the other groups were used to establish a model of stage III/IV pressure ulcers.After successful modeling，the rats in the blank group and the model group were given irrigation with 0.9% sodium chloride injection，those in the Shirun Shaoshang ointment group were given the external application of Shirun Shaoshang ointment，and those in the Xiangpi Shengji ointment group were given the external application of Xiangpi Shengji ointment.Intervention was given for 14 days.Related indicators were observed on days 3，7，and 14 of intervention，including wound healing rate，histopathology，the levels of interleukin-6 (IL-6)，tumor necrosis factor-α (TNF-α)，and vascular endothelial growth factor (VEGF)，and the protein expression levels of p38 mitogen-activated protein kinase (p38MAPK) and MAPK-activated protein kinase 2 (MK2).Results：Compared with the model group，the Shirun Shaoshang ointment group and Xiangpi Shengji ointment group had a significant increase in wound healing rate on days 3，7，and 14 of treatment (P<0.05).Histopathology results showed that on day 14 of treatment，the model group had a small number of capillaries，while the Shirun Shaoshang ointment group and Xiangpi Shengji ointment group had a large number of fibroblasts and abundant capillaries.On days 3，7，and 14 of treatment，there were significant differences in the content of IL-6，TNF-α，VEGF，p38MAPK protein，and MK2 protein between the Shirun Shaoshang ointment/Xiangpi Shengji ointment group and the model group (P<0.05).There were significant differences between the Xiangpi Shengji ointment group and the Shirun Shaoshang ointment group in the content of IL-6 on days 7 and 14 and the content of TNF-α and VEGF on day 7 (P<0.05).Conclusion：Xiangpi Shengji ointment can promote wound repair of pressure ulcers，possibly by inhibiting the protein expression of p38MAPK and MK2，reducing the release of IL-6 and TNF-α，and regulating the synthesis of VEGF. |
| Key words: pressure ulcers Xiangpi Shengji ointment rat interleukin-6 tumor necrosis factor-α vascular endothelial growth factor p38 mitogen-activated protein kinase MAPK-activated protein kinase 2 |
