| 摘要: |
| 目的：研究蠲痹历节清方对高尿酸血症模型大鼠的干预作用及机制。方法：将60只雄性SD大鼠随机分为空白组,模型组,苯溴马隆组,蠲痹历节清方低、中、高剂量组,每组各10只。采用连续8d氧嗪酸钾100 mg/kg腹腔注射和次黄嘌呤500 mg/kg灌胃的方法复制高尿酸血症大鼠模型。实验第4天给予造模剂30 min后,各组分别予对应治疗药物干预,连续5 d。检测各组血尿酸（SUA）、血肌酐（SCr）和血尿素氮（BUN）含量,并观察肾脏组织病理变化。结果：与空白组比较,模型组大鼠SUA、BUN升高（P<0.05）,肾脏病理改变明显,提示造模成功;与模型组比较,蠲痹历节清方低、中、高剂量组SUA、BUN均降低,且随着剂量升高,SUA水平下降越明显（P<0.05）,各给药组大鼠肾脏组织病理改变较模型组均有不同程度的改善;各组SCr水平组间比较,差异无统计学意义（P>0.05）。结论：蠲痹历节清方有明显的降尿酸作用,可改善模型大鼠肾脏组织病理损伤,保护肾功能,且降尿酸作用与肾损伤的治疗效果随着给药剂量的增加而越显著。 |
| 关键词: 高尿酸血症 蠲痹历节清方 血尿酸 血肌酐 血尿素氮 实验研究 |
| DOI： |
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| Intervention effect of Juanbi Lijieqing prescription on a rat model of hyperuricemia：An experimental study |
| WANG Yu,XIONG Hui,XIAO Wenzhong |
| (Hunan University of Chinese Medicine,Changsha 410208,Hunan,China) |
| Abstract: |
| Objective：To investigate the intervention effect of Juanbi Lijieqing prescription on a rat model of hyperuricemia and related mechanism.Methods：A total of 60 male Sprague-Dawley rats were randomly divided into blank group,model group,benzbromarone group,and low-,middle-,and high-dose Juanbi Lijieqing prescription groups,with 10 rats in each group.A rat model of hyperuricemia was established by intraperitoneal injection of oteracil potassium 100 mg/kg and intragastric administration of hypoxanthine 500 mg/kg for 8 consecutive days.At 30 minutes after the modeling agents were given on day 4 of the experiment,each group was given the corresponding drug intervention for 5 consecutive days.Serum uric acid (SUA),serum creatinine (SCr),and blood urea nitrogen (BUN) were measured for each group,and renal histopathological changes were also observed.Results：Compared with the blank group,the model group had significant increases in SUA and BUN (P<0.05) and significant renal histopathological changes,suggesting that the model was established successfully.Compared with the model group,the low-,middle-,and high-dose Juanbi Lijieqing prescription groups had significant reductions in SUA and BUN,and the degree of reduction in SUA increased with the increase in dose (P<0.05).Compared with the model group,the administration groups had varying degrees of improvement in renal histopathological changes.There was no significant difference in the level of SCr between groups (P>0.05).Conclusion：Juanbi Lijieqing prescription has a marked effect in reducing uric acid and can improve renal histopathological changes and protect renal function in model rats,and such uric acid-lowering effect and therapeutic effect on renal injury increase with dose. |
| Key words: hyperuricemia Juanbi Lijieqing prescription serum uric acid serum creatinine blood urea nitrogen experimental study |
