| 摘要: |
| 目的：探讨天降血栓通丸对Apo-/-小鼠动脉粥样硬化的干预作用及其机制。方法：将30只动脉粥样硬化建模成功的ApoE-/-小鼠随机分为模型组、阿托伐他汀组和天降血栓通丸组，每组各10只，分别给予0.9%氯化钠注射液、阿托伐他汀、天降血栓通丸药液灌胃8周。10只C57BL/6小鼠予普通饲料喂养，作为对照组，灌胃0.9%氯化钠注射液。干预结束后，检测血清总胆固醇（TC）、三酰甘油（TG）、低密度脂蛋白胆固醇（LDL-C）、高密度脂蛋白胆固醇（HDL-C）、单核细胞趋化因子-1（MCP-1）、细胞间黏附分子-1（ICAM-1）、血管细胞黏附分子-1（VCAM-1）水平，检测主动脉组织c-Jun氨基末端活化蛋白激酶（JNK）、丝裂原活化蛋白激酶（p38MAPK）mRNA的表达。结果：与模型组比较，阿托伐他汀组和天降血栓通丸组血清TG、TC、LDL-C、MCP-1、ICAM-1、VCAM-1水平及主动脉组织JNK mRNA、p38MAPK mRNA表达均降低，差异均有统计学意义（P<0.05）；与对照组比较，模型组血清TG、TC、LDL-C、MCP-1、ICAM-1、VCAM-1水平及主动脉组织JNK mRNA、p38MAPK mRNA表达水平均升高，差异均有统计学意义（P<0.05）。结论：天降血栓通丸具有防治动脉粥样硬化的作用，其机制可能与抑制JNK/p38 MAPK信号，下调MCP-1、ICAM-1、VCAM-1表达，减轻炎症反应有关。 |
| 关键词: 动脉粥样硬化 天降血栓通丸 ApoE基因敲除小鼠 实验研究 |
| DOI： |
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| Interventional effect of Tianjiang Xueshuantong pills on atherosclerosis in ApoE-/- mice and its mechanism |
| WANG Juping,ZHAO Congna,ZHANG Baonan |
| (Beichen Hospital of Traditional Chinese Medicine，Tianjin 300400，China) |
| Abstract: |
| Objective：To investigate the interventional effect of Tianjiang Xueshuantong pills on atherosclerosis in ApoE-/- mice and its mechanism.Methods：A total of 30 ApoE-/- mice with successful atherosclerosis modeling model,which were randomly divided into model group，atorvastatin group，and Tianjiang Xueshuantong pill group，with 10 mice in each group.The above three groups were given 0.9% sodium chloride injection，atorvastatin solution，and Tianjiang Xueshuantong pill solution，respectively，by gavage for 8 weeks.A total of 10 C57BL/6 mice fed with normal diet were selected as control group and were given 0.9% sodium chloride injection by gavage.After intervention，the serum levels of total cholesterol (TC)，triglyceride (TG)，low-density lipoprotein cholesterol (LDL-C)，high-density lipoprotein cholesterol，monocyte chemoattractant protein-1(MCP-1)，intercellular adhesion molecule-1 (ICAM-1)，and vascular cell adhesion molecule-1 (VCAM-1) were measured，as well as the mRNA expression of c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (p38 MAPK) in aortic tissue.Results：Compared with the model group，the atorvastatin group and the Tianjiang Xueshuantong pill group had significant reductions in the serum levels of TG，TC，LDL-C，MCP-1，ICAM-1，and VCAM-1 and the mRNA expression of JNK and p38 MAPK in aortic tissue (P<0.05).Compared with the control group，the model group had significant increases in the serum levels of TG，TC，LDL-C，MCP-1，ICAM-1，and VCAM-1 and the mRNA expression of JNK and p38 MAPK in aortic tissue (P<0.05).Conclusion：Tianjiang Xueshuantong pills have a certain effect in the prevention and treatment of atherosclerosis，possibly by inhibiting the JNK/p38 MAPK signal，downregulating the expression of MCP-1，ICAM-1，and VCAM-1，and alleviating inflammatory response. |
| Key words: atherosclerosis Tianjiang Xueshuantong pills ApoE-/-mouse experimental study |
