| 摘要: |
| 目的：运用网络药理学技术探讨“黄芪-桂枝”治疗慢性心力衰竭（CHF）的作用机制。方法：通过中药系统药理学数据库与分析平台（TCMSP）筛选出“黄芪-桂枝”中潜在活性化合物及其潜在作用靶点，采用Gene Cards、OMIM数据库获得相关疾病靶点，通过String数据库构建关键靶点蛋白相互作用网络，使用Metascape在线工具对共同靶点基因进行GO功能和KEGG通路富集分析。并将相关数据导入Cytoscape 3.6.1软件进行可视化分析，构建“活性成分-CHF-靶点”网络模型和“靶点-GO-KEGG”网络模型。结果：筛选出27个主要活性成分，共涉及207个中药有效成分和175个中药-疾病关键靶点。“黄芪-桂枝”主要通过调控丝氨酸/苏氨酸蛋白激酶1（AKT1）、丝裂原活化蛋白激酶-1（MAPK1）、白细胞介素-6（IL-6）、转录因子Ap-1（JUN）、肿瘤坏死因子（TNF）等蛋白，以及参与癌症途径、乙型肝炎信号通路、磷脂酰肌醇3-激酶(PI3K)-蛋白激酶B(Akt)信号通路、癌症蛋白多糖通路、人类T淋巴细胞瘤病毒Ⅰ型(HTLV-I)感染通路等发挥治疗CHF的作用。结论：本研究揭示了“黄芪-桂枝”组分中的有效化合物以多靶点、多通路、多途径治疗CHF的作用机制，为临床研究提供了新思路。 |
| 关键词: 慢性心力衰竭 黄芪 桂枝 网络药理学 作用机制 |
| DOI： |
|
|
| Mechanism of action of Astragalus membranaceus-Ramulus Cinnamomi in treatment of chronic heart failure based on network pharmacology |
| PENG Liqi,TANG Yanping,CAI Huzhi |
| (Hunan University of Chinese Medicine，Changsha 410208，Hunan，China;The First Affiliated Hospital of Hunan University of Chinese Medicine，Changsha 410007，Hunan，China) |
| Abstract: |
| Objective：To investigate the mechanism of action of Astragalus membranaceus-Ramulus Cinnamomi in the treatment of chronic heart failure (CHF) based on network pharmacology.Methods：The Traditional Chinese Medicine Systems Pharmacology platform was used to screen out the potential active components of Astragalus membranaceus-Ramulus Cinnamomi and their potential action targets；the Gene Cards and OMIM databases were used to obtain the disease targets；the String database was used to construct a protein-protein interaction network of key targets；the Metascape online tool was used to perform GO enrichment analysis and KEGG pathway enrichment analysis.Related data were imported into Cytoscape 3.6.1 for visual analysis，and an “active component-CHF-target” network model and a “target-GO-KEGG” network model were established.Results：A total of 27 main active components were screened out，which involved 207 effective constituents and 175 key traditional Chinese medicine-disease targets.Astragalus membranaceus-Ramulus Cinnamomi exerted a therapeutic effect on CHF mainly by regulating the proteins including RAC-alpha serine/threonine-protein kinase，mitogen-activated protein kinase 1，interleukin-6，transcription factor Ap-1，and tumor necrosis factor and participating in the cancer pathway，the hepatitis B signaling pathway，the phosphoinositide 3-kinase-protein kinase B signaling pathway，the cancer proteoglycan pathway，and the HTLV-I infection pathway.Conclusion：This study revealed the mechanism of action of the effective compounds in the "Astragalus-Guizhi" component in the treatment of CHF with multiple targets, multiple pathways, and multiple ways, providing a new idea for clinical research. |
| Key words: chronic heart failure Astragalus membranaceus Ramulus Cinnamomi network pharmacology mechanism of action |
