| 摘要: |
| 目的：研究柴胡疏肝散合济川煎对肝郁气滞合并阳虚型便秘型肠易激综合征（IBS-C）模型大鼠的治疗效果及作用机制。方法：将56只SPF级雄性SD大鼠随机分为正常组（8只）和模型组（48只），模型组采用冰水灌胃、限制饮水结合慢性束缚应激的方法制备肝郁气滞合并阳虚型IBS-C大鼠模型。将造模成功的大鼠随机分为模型对照组、复方聚乙二醇电解质散（PEG-EP)组、黛力新组及中药低、中、高剂量组，每组各8只。各组进行药物干预14 d。观察指标包括大鼠一般状态、糖水偏好率、旷场实验评分、内脏敏感性、小肠推进率、结肠黏膜HE染色及血清5-羟色胺( 5-HT) 、生长抑素(SS) 、血管活性肠肽（VIP）含量测定。结果：与模型对照组相比，柴胡疏肝散合济川煎能使模型大鼠的粪便粒数、粪便含水量、Bristol 分值、内脏敏感性、旷场试验评分、糖水偏好百分比、小肠推进率增加(P<0.01 或P<0.05)，血清5-HT、SS含量降低(P<0.05)，血清VIP含量升高（P<0.05）;与PEG-EP组、黛力新组相比，中药高剂量组大鼠血清5-HT、SS均降低(P<0.05)，VIP水平升高(P<0.05)。结论：大鼠肝郁气滞合并阳虚型IBS-C模型制备成功，柴胡疏肝散合济川煎可改善模型大鼠肝郁和便秘症状，其作用机制可能与降低大鼠血清5-HT、SS含量和升高大鼠血清VIP含量有关。 |
| 关键词: IBS-C 肝郁气滞合并阳虚证 柴胡疏肝散合济川煎 VIP 5-HT SS |
| DOI： |
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| Effect of Chaihu Shugan powder combined with Jichuan decoction in improving the symptoms of rats with constipation-predominant irritable bowel syndrome：An experimental study |
| GAO Hongzhi,YANG Yongjun,LIU Shihong |
| (Affiliated Hospital of Chuanbei Medical College，Nanchong 637000，Sichuan，China) |
| Abstract: |
| Objective：To investigate the therapeutic effect and mechanism of action of Chaihu Shugan powder combined with Jichuan decoction in a rat model of constipation-predominant irritable bowel syndrome (IBS-C) with liver Qi stagnation and Yang deficiency. Methods：A total of 56 specific pathogen-free male Sprague-Dawley rats were randomly divided into normal group with 8 rats and model group with 48 rats，and the rats in the model group were treated with ice water by gavage，restricted water intake，and chronic restraint stress to establish a model of IBS-C with liver Qi stagnation and Yang deficiency. After successful modeling，the rats were randomly divided into model control group，polyethylene glycol electrolyte powder (PEG-EP) group，Deanxit group，and low-，middle-，and high-dose traditional Chinese medicine (TCM) groups，with 8 rats in each group. Drug intervention was given for 14 days for each group. Observation indicators included general status，rate of sugar water preference，score of the open field test，visceral sensitivity，small intestine propulsion rate，HE staining of colonic mucosa，and content determination of 5-hydroxytryptamine (5-HT)，somatostatin (SS)，and vasoactive intestinal peptide (VIP). Results：Compared with the model control group，the Chaihu Shugan powder+Jichuan decoction group had significant increases in the number of fecal pellets，fecal water content，Bristol score，visceral sensitivity，score of the open field test，rate of sugar water preference，and small intestine propulsion rate (P<0.01 or P<0.05)，significant reductions in the content of serum 5-HT and SS (P<0.05)，and a significant increase in serum VIP (P<0.05). Compared with the PEG-EP group and the Deanxit group，the high-dose TCM group had significant reductions in serum 5-TH and SS (P<0.05) and a significant increase in the level of VIP (P<0.05). Conclusion：A rat model of IBS-C with liver Qi stagnation and Yang deficiency is established successfully. Chaihu Shugan powder combined with Jichuan decoction can improve stagnation of liver Qi and the symptom of constipation，possibly by reducing the serum levels of 5-HT and SS and increasing the serum level of VIP. |
| Key words: constipation-predominant irritable bowel syndrome liver Qi stagnation and Yang deficiency Chaihu Shugan powder combined with Jichuan decoction vasoactive intestinal peptide 5-hydroxytryptamine somatostatin |
