| 摘要: |
| 目的：基于网络药理学预测桃仁-红花治疗葡萄胎（HM）的分子作用机制。方法：以网络药理学方法为基础，依托中药系统药理学数据库和分析平台（TCMSP）筛选桃仁-红花的活性成分及作用靶点；借助GeneCards、OMIM、Drug Bank、PharmGKB等数据库筛选葡萄胎的疾病靶标，绘制Venn图，构建药对-疾病“活性成分-作用靶点”网络图，绘制蛋白互作网络（PPI），完成GO和 KEGG 富集分析，进一步用分子对接来验证和筛选靶点。结果：研究共筛选得到槲皮素、木犀草素等8个活性成分，分别作用于AKT1、TP53、EGFR等39个相关靶点；GO富集分析显示桃仁-红花的活性成分主要涉及凝血、改善血流动力学、调控细胞凋亡和细胞增殖、抗炎等生物学过程。KEGG富集通路HM有显著作用的有MAPK信号通路、血流剪切应力与动脉粥样硬化信号通路等；分子对接进一步验证了槲皮素与AKT1的相关性。结论：桃仁-红花药对包括槲皮素、木犀草素等8个活性成分，其治疗葡萄胎主要通过多靶点多通路起作用，为后续临床治疗葡萄胎提供了新的思路。 |
| 关键词: 桃仁 红花 葡萄胎 妊娠滋养细胞疾病 网络药理学 |
| DOI： |
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| Active components of peach seed-Carthamus tinctorius and its molecular mechanism in treatment of hydatidiform mole：An analysis based on network pharmacology |
| XIA Yu,ZHAO Yudong,KUANG Gaoyan |
| (Hunan University of Chinese Medicine，Changsha 410208，Hunan，China;The Affiliated Hospital of Chengdu University of Traditional Chinese Medicine，Chengdu 610075，Sichuan，China;The Affiliated Hospital of Chengdu University of Traditional Chinese Medicine，Changsha 410007，Hunan，China) |
| Abstract: |
| Objective：To investigate the molecular mechanism of peach seed-Carthamus tinctorius in the treatment of hydatidiform mole (HM) based on network pharmacology.Methods：Based on the method of network pharmacology，the Traditional Chinese Medicine Systems Pharmacology platform was used to screen out the active components of peach seed-Carthamus tinctorius and related targets.GeneCards，OMIM，Drug Bank，and PharmGKB databases were used to screen out the targets of HM.Venn diagram was plotted，the “drug pair-disease-active component-target” network was constructed，the protein-protein interaction network was established，and GO and KEGG enrichment analyses were performed.Molecular docking was used for the validation and screening of targets.Results：A total of 8 active components (including quercetin and luteolin) were screened out，which acted on 39 targets including AKT1，TP53，and EGFR.The GO enrichment analysis showed that the active components of peach seed-Carthamus tinctorius were mainly involved in the biological processes such as coagulation，improvement of hemodynamics，regulation of cell apoptosis and cell proliferation，and anti-inflammation.The KEGG pathway enrichment analysis showed that the signaling pathways of MAPK，blood shear stress，and atherosclerosis had a marked effect on HM，and molecular docking further verified the association between quercetin and AKT1.Conclusion：The peach seed-Carthamus tinctorius drug pair has 8 active components including quercetin and luteolin and exerts a therapeutic effect on HM via multiple targets and pathways，which provides new ideas for the clinical treatment of HM. |
| Key words: peach seed Carthamus tinctorius hydatidiform mole gestational trophoblastic disease network pharmacology |
