| 摘要: |
| 目的：观察洛哌丁胺对大鼠慢传输型便秘模型稳定性的影响。方法：将20只大鼠随机分成对照组、模型组、模型维持组、治疗组,每组各5只。模型组、模型维持组、治疗组予洛哌丁胺混悬液灌胃,对照组以等体积的0.9%氯化钠注射液灌胃,连续6 d。造模第7天开始模型维持组继续予洛哌丁胺混悬液灌胃,治疗组灌胃莫沙必利混悬液,对照组及模型组灌胃等体积0.9%氯化钠注射液。观察大鼠造模前、造模6d和干预6d后的一般情况、6 h粪便粒数及重量、粪便含水率及大肠墨汁推进率。结果：造模6 d后大鼠出现活动减少,采食减少,毛发枯萎脱落,尿黄,大便数量及含水量减少;模型组造模停止6 d后及治疗组干预后大鼠一般情况改善,采食量、体质量增长,粪便粒数、粪便含水率均增加。各组大鼠6 h粪便粒数、重量、含水量方面,造模6d后,模型组、模型维持组、治疗组与对照组组间比较及造模前后组内比较,差异均有统计学意义（P<0.05）;对照组、模型组、治疗组与模型维持组比较,差异均有统计学意义P<0.05）;治疗组、模型维持组、模型组干预6d后与造模6d后比较,差异均有统计学意义（P<0.05）。对照组、模型组、治疗组与模型维持组大鼠大肠墨汁推进率比较,差异均有统计学意义（P＜0.05）。结论：洛哌丁胺大鼠慢传输型便秘模型是一种相对简便的慢传输型便秘大鼠模型,模型停药后可自愈,需用药物维持模型的稳定性。 |
| 关键词: 慢传传输型便秘 动物模型 大鼠 洛哌丁胺 实验研究 |
| DOI： |
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| Effect of loperamide on the stability of the rat model of slow transit constipation：An experimental study |
| HUANG Yajuan,JIANG Shisheng,XIANG Yanfang |
| (The Affiliated Hospital of Hunan Academy of Chinese Medicine,Changsha 410006,Hunan,China;Hunan University of Chinese Medicine,Changsha 410208,Hunan,China) |
| Abstract: |
| Objective：To investigate the effect of loperamide on the stability of the rat model of slow transit constipation.Methods：A total of 20 rats were randomly divided into control group,model group,model maintenance group,and treatment group,with 5 rats in each group.The rats in the model group,the model maintenance group,and the treatment group were given loperamide suspension by gavage,and those in the control group were given an equal volume of 0.9% sodium chloride injection,for 6 consecutive days.Since day 7 of modeling,the rats in the model maintenance group were given loperamide suspension by gavage,those in the treatment group were given mosapride suspension by gavage,and those in the control group and the model group were given an equal volume of 0.9% sodium chloride injection.General status,number and weight of fecal pellets at 6 hours,fecal water content,and ink propelling rate in the large intestine were observed before modeling,after 6 days of modeling,and after 6 days of intervention.Results：After 6 days of modeling,the rats showed reductions in activity and ingestion,withered hairs,yellow urine,and reductions in the number of fecal pellets and fecal water content;improvement in general status and increases in food intake,body weight,number of fecal pellets,and fecal water content were observed in the model group on day 6 after the modeling ended and in the treatment group after intervention.After 6 days of modeling,the model group,the model maintenance group,the treatment group,and the control group had significant changes in the number and weight of fecal pellets at 6 hours and fecal water content,and there were significant differences between these groups (P＜0.05),and there were also significant differences between the control group/model group/treatment group and the model maintenance group (P＜0.05);the treatment group,the model maintenance group,and the model group had significant changes in these indices from the end of 6 days of modeling to the end of 6 days of intervention (P＜0.05).There was a significant difference in ink propelling rate of the large intestine between the control group/model group/treatment group and the model maintenance group(P＜0.05).Conclusion：A rat model of slow transit constipation based on loperamide is a relatively simple and convenient model,with self-healing after drug withdrawal,and therefore,drugs are needed to maintain the stability of the model. |
| Key words: slow transit constipation animal model rat loperamide experimental study |
