| 摘要: |
| 目的：探讨加味丹参饮预处理通过抑瘤基因/磷脂酰肌醇-3激酶/蛋白激酶B（PTEN/PI3K/Akt）信号通路对大鼠心肌缺血再灌注损伤（IRI）的保护作用及机制。方法：将75只雄性远交群（SD）大鼠随机分为5组：假手术组、模型组、加味丹参饮组、加味丹参饮+PI3K/Akt通路抑制剂（LY294002）组、LY294002组，采用结扎大鼠冠状动脉左前降支30min后再灌注60min的方法制备IRI模型。采用酶联免疫吸附测定（ELISA）法检测各组大鼠心肌肌钙蛋白I（cTnI）表达；取心肌梗死边缘区心肌组织，采用脱氧核糖核苷酸末端转移酶介导的缺口末端标记（TUNEL）法检测心肌凋亡指数，采用免疫组化法检测各组大鼠心肌组织Akt、PTEN的表达。结果：与假手术组比较，模型组cTnI水平显著升高，大鼠心肌凋亡指数增加，PTEN、Akt表达均显著升高（P<0.05）；与模型组比较，加味丹参饮组cTnI水平均显著降低，Akt表达升高，心肌凋亡指数和PETN表达降低（P<0.05）；与加味丹参饮组比较，加入抑制剂后，cTnI水平均明显降低，Akt表达降低，PTEN表达升高（P<0.05）。结论：大鼠心肌缺血再灌注时，加味丹参饮可以通过降低PTEN表达，激活PI3K/Akt信号通路，抑制大鼠心肌细胞的凋亡，从而减轻心肌损伤，保护心肌。 |
| 关键词: 加味丹参饮 PI3K/Akt PTEN 心肌缺血再灌注损伤 实验研究 |
| DOI： |
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| Jiawei Danshen decoction inhibits ischemia-reperfusion injury via the PTEN/PI3K/Akt signaling pathway |
| WU Ruoxia,LI Zhengyang,BIAN Weisi |
| (Hunan University of Chinese Medicine，Changsha 410208，Hunan，China) |
| Abstract: |
| Objective：To investigate the protective effect of Jiawei Danshen decoction pretreatment against myocardial ischemia-reperfusion injury (IRI) in rats via the PTEN/PI3K/Akt signaling pathway and related mechanisms.Methods：A total of 75 male outbred Sprague-Dawley rats were randomly divided into sham-operation group，model group，Jiawei Danshen decoction group，Jiawei Danshen decoction+PI3K/Akt pathway inhibitor (LY294002) group，and LY294002 group.A rat model of IRI was established by ligation of the left anterior descending coronary artery，followed by 60-minute reperfusion after 30 minutes.ELISA was used to measure the expression of cardiac troponin I (cTnI).Myocardial tissue was collected from the infarct border zone；terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling was used to measure myocardial apoptotic index and immunohistochemistry was used to measure the expression of Akt and PTEN in myocardial tissue.Results：Compared with the sham-operation group，the model group had significant increases in cTnI，myocardial apoptotic index，and expression of PTEN and Akt (P<0.05).Compared with the model group，the Jiawei Danshen decoction group had a significant reduction in cTnI，a significant increase in the expression of Akt，and significant reductions in myocardial apoptotic index and PTEN (P<0.05).Compared with the Jiawei Danshen decoction，the Jiawei Danshen decoction+LY294002 group had significant reductions in cTnI and Akt and a significant increase in PTEN (P<0.05).Conclusion：In a rat model of myocardial IRI，Jiawei Danshen decoction can alleviate myocardial injury and protect the myocardium by reducing the expression of PTEN，activating the PI3K/Akt signaling pathway，and inhibiting the apoptosis of cardiomyocytes. |
| Key words: Jiawei Danshen decoction PI3K/Akt PTEN myocardial ischemia-reperfusion injury experimental study |
