| 摘要: |
| 目的：研究苦参汤对皮肤溃疡大鼠伤口愈合的影响。方法：通过皮下注射75%冰醋酸诱导大鼠皮肤溃疡模型，将模型大鼠分为5组，G1采用蒸馏水干预，G2～5分别采用苦参汤低、中、高剂量及0.02%高锰酸钾溶液（PP）进行局部治疗，连续7d。治疗后对各组溃疡面积、溃疡评分和组织学评估，通过免疫组织化学分析检测前炎性细胞因子前列腺素E2（PGE-2）和白细胞介素-8（IL-8）的表达。结果：苦参汤对皮肤溃疡大鼠创口愈合有促进作用，中剂量和高剂量苦参汤治疗的大鼠溃疡愈合更佳（P<0.05），低剂量苦参汤治疗的肉眼可见溃疡评分显著降低（P<0.01），PP组在降低溃疡面积和肉眼可见的溃疡评分方面，差异无统计学意义（P>0.05）；所有剂量的苦参汤和PP均改善了大鼠诱导的皮肤溃疡的组织学损伤；高剂量苦参汤和PP使大鼠皮肤溃疡组织中的PGE-2和IL-8表达显著降低（P<0.01）；各组大鼠均未发现与治疗有关的毒性。结论：苦参汤可促进皮肤溃疡大鼠的伤口愈合，其对PGE-2和IL-8的抑制是增强伤口愈合的可能作用机制。 |
| 关键词: 皮肤溃疡 苦参汤 细胞因子 实验研究 |
| DOI： |
|
|
| Effect of Kushen decoction on wound healing in rats with skin ulcer |
| LIU Haiyan,WU Zhe |
| (The Affiliated Hospital of Hunan Academy of Chinese Medicine，Changsha 410006，Hunan，China) |
| Abstract: |
| Objective：To investigate the effect of Kushen decoction on wound healing in rats with skin ulcer.Methods：Subcutaneous injection of 75% glacial acetic acid was given to establish a rat model of skin ulcer，and then the model rats were divided into groups 1-5.The rats in group 1 were given distilled water，and those in groups 2～5 were given local treatment with low-，middle-，and high-dose Kushen decoction and 0.02% potassium permanganate (PP) solution，respectively.The course of treatment was 7 consecutive days.Ulcer area，ulcer score，and histology were evaluated after treatment，and immunohistochemistry was used to measure the expression of the proinflammatory cytokines prostaglandin E2(PGE2) and interleukin-8 (IL-8).Results：Kushen decoction promoted wound healing in rats with skin ulcer，and middle- and high-dose Kushen decoction had a better effect (P<0.05)，while low-dose Kushen decoction significantly reduced macroscopic ulcer score (P<0.01).The group treated with PP had reductions in ulcer area and macroscopic ulcer score (P>0.05).Low-，middle-，and high-dose Kushen decoction and PP improved the histological injury of skin ulcer.High-dose Kushen decoction and PP significantly reduced the expression of PGE-2 and IL-8 in skin ulcer tissue (P<0.01).No treatment-related toxicity was observed.Conclusion：Kushen decoction can promote wound healing in rats with skin ulcer，possibly by inhibiting PGE-2 and IL-8. |
| Key words: skin ulcer Kushen decoction cytokine experimental study |
