| 摘要: |
| 目的：探讨臭牡丹黄酮类化合物通过调控CXCR4对A549转移能力的影响。方法：采用siRNA技术将A549细胞CXCR4基因沉默后，设立实验分组，为A549对照组、A549+臭牡丹黄酮组、A549-CXCR4沉默组、A549-CXCR4沉默+臭牡丹黄酮组。每组加入100ng/ml的SDF-1处理48h后，Transwell检测各组A549细胞侵袭能力，Western Blot法检测各组CXCR4、MMP-9蛋白表达情况。结果：与A549对照组相比，加入臭牡丹黄酮后A549其体外侵袭能力下降，沉默CXCR4后，A549细胞侵袭能力显著下降，差异均有统计学意义（P<0.01）；与A549对照组相比，A549+臭牡丹黄酮组能降低CXCR4和MMP-9的表达，A549-CXCR4沉默组与A549-CXCR4沉默+臭牡丹黄酮组CXCR4和MMP-9的表达明显降低。结论：臭牡丹黄酮类化合物通过下调CXCR4表达，降低MMP-9蛋白表达，进而降低A549侵袭能力可能是其治疗肺癌的作用机制之一。 |
| 关键词: 肺癌 臭牡丹黄酮类化合物 CXCR4 A549 |
| DOI： |
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| Flavonoids in Clerodendrum bungei affects the metastasis of A549 cells by regulating CXCR4 |
| TAN Xiaoning,LI Yongmin,YU Na |
| (The Affiliated Hospital of Hunan Academy of Chinese Medicine，Changsha 410006，Hunan，China) |
| Abstract: |
| Objective：To investigate the effect of flavonoids in Clerodendrum bungei on the metastasis of A549 cells via the regulation of CXCR4.Methods：A549 cells with the CXCR4 gene silenced by the siRNA technique were divided into A549 control group，A549+Clerodendrum bungei flavonoids group，A549-CXCR4 silencing group，and A549-CXCR4 silencing+Clerodendrum bungei flavonoids group.After the treatment with SDF-1 100ng/ml for 48 hours，Transwell assay was used to evaluate the invasion ability of A549 cells，and Western blot was used to measure the protein expression of CXCR4 and matrix metallopeptidase-9（MMP-9）.Results：Compared with the A549 control group，the A549 cells treated with Clerodendrum bungei flavonoids had a reduction in in vitro invasion ability，and after CXCR4 silencing，the A549 cells had a significant reduction in invasion ability （P<0.01）.Compared with the A549 control group，the A549+Clerodendrum bungei flavonoids group had significant reductions in the expression of CXCR4 and MMP-9，and the A549-CXCR4 silencing group and the A549-CXCR4 silencing+Clerodendrum bungei flavonoids group also had significant reductions in the expression of CXCR4 and MMP-9.Conclusion：Clerodendrum bungei flavonoids can reduce the invasion ability of A549 cells by downregulating CXCR4 and reducing the protein expression of MMP-9 and thus exerts a therapeutic effect on lung cancer. |
| Key words: lung cancer Clerodendrum bungei flavonoid CXCR4 A549 |
