| 摘要: |
| 目的：观察左归丸对去势大鼠血清中基质金属蛋白酶-2（MMP-2）、基质金属蛋白酶-9（MMP-9）和基质金属蛋白酶-13（MMP-13）的影响，探讨左归丸抗骨质疏松的作用机制。方法：将48只6月龄雌性SD大鼠随机分为模型组、正常组、西药组、中药低剂量组、中药高剂量组和假手术组。除假手术组外，其余组均采用双侧切除大鼠卵巢的方法制备去势大鼠，造模后进行治疗干预，持续3个月。ELISA法检测血清中MMP-2、MMP-9和MMP-13的含量。结果：与假手术组相比，模型组血清中MMP-2、MMP-9和MMP-13含量均有不同程度的升高(P<0.05)；与模型组相比，西药组和中药高剂量组血清中MMP-2、MMP-9和MPP-13含量均有不同程度的下降(P<0.05)。结论：左归丸可通过降低去势大鼠血清中的MMP-2、MMP-9和MMP-13的含量，从而抑制骨细胞的功能活性，降低骨质的吸收破坏。 |
| 关键词: 左归丸 去势大鼠 基质金属蛋白酶 MMP-2 MMP-9 MMP-13 |
| DOI： |
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| Effect of Zuogui pills on serum matrix metalloproteinases in ovariectomized rats |
| TANG Hao,YU Na,QIU Xiangzhong |
| (The Affiliated Hospital of Hunan Academy of Chinese Medicine，Changsha 410006，Hunan，China;Hunan University of Chinese Medicine，Changsha 410208，Hunan，China) |
| Abstract: |
| Objective：To investigate the effect of Zuogui pills on the serum levels of matrix metalloproteinase-2 (MMP-2)，matrix metalloproteinase-9 (MMP-9)，and matrix metalloproteinase-13 (MMP-13) in ovariectomized rats，as well as the mechanism of action of Zuogui pills in anti-osteoporosis activities.Methods：A total of 48 female Sprague-Dawley rats aged 6 months were randomly divided into model group，normal group，Western medicine group，low- and high-dose traditional Chinese medicine (TCM) groups，and sham-operation group.All rats except those in the sham-operation group were given resection of both ovaries to obtain ovariectomized rats，and treatment was given for 3 consecutive months after modeling.ELISA was used to measure the serum levels of MMP-2，MMP-9，and MMP-13.Results：Compared with the sham-operation group，the model group had varying degrees of increases in the serum levels of MMP-2，MMP-9，and MMP-13 (P<0.05).Compared with the model group，the Western medicine group and the high-dose TCM group had varying degrees of reductions in the serum levels of MMP-2，MMP-9，and MMP-13 (P<0.05).Conclusion：Zuogui pills can inhibit the functional activity of bone cells and reduce bone absorption and destruction by reducing the serum levels of MMP-2，MMP-9，and MMP-13 in ovariectomized rats. |
| Key words: Zuogui pills ovariectomized rat matrix metalloproteinase matrix metalloproteinase-2 matrix metalloproteinase-9 matrix metalloproteinase-13 |
