| 摘要: |
| 目的：通过观察益气活血化痰法对动脉粥样硬化（AS）模型大鼠核因子κB（NF-κB）信号通路表达的影响，阐明护心康片抗动脉粥样硬化的部分作用机制。方法：采用注射大剂量维生素D3注射液及喂养高脂饲料法制备大鼠动脉粥样硬化模型，随机分为护心康片高、中、低（1.64g/kg、0.82g/kg、0.41g/kg）剂量组，阳性对照组（阿托伐他汀钙片，2mg/kg），模型组（蒸馏水，10ml/kg）和空白组。治疗12周后处死，采用双抗体夹心酶联免疫分析法（ELISA）检测血清炎症因子白介素-1β（IL-1β）、白介素-6（IL-6）、肿瘤坏死因子（TNF-α）的表达水平；取动脉组织采用蛋白免疫印记法（Western blotting）检测NF-κB信号通路的蛋白表达量，用图像分析系统分析其灰度值，以目的蛋白与β-acting内参蛋白的比值来表示组织蛋白中NF-κB的表达量。结果：与模型组比较，护心康片中、高剂量组及阳性对照组的血清内炎症因子IL-1β、IL-6、TNF-α水平与NF-κB信号通路蛋白的表达水平均显著降低（P<0.01）。结论：益气活血化痰法通过下调NF-κB信号通路蛋白的表达水平，阻断该信号通路的活化，从而抑制模型大鼠炎症因子IL-1β、IL-6、TNF-α的释放，达到抗动脉粥样硬化的作用。 |
| 关键词: 动脉粥样硬化 大鼠 益气活血化痰法 NF-κB信号通路 实验研究 |
| DOI： |
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| Effect of Qi-tonifying，blood-activating，and phlegm-eliminating therapy on the NF-κB signaling pathway in rats with atherosclerosis：An experimental study |
| WANG Kaili，,YU Zhengke，,WANG Qingxiang |
| (The Affiliated Hospital of Hunan Academy of Chinese Medicine，Changsha 410006，Hunan，China;Hunan University of Chinese Medicine，Changsha 410208，Hunan，China) |
| Abstract: |
| Objective：To investigate the effect of Qi-tonifying，blood-activating，and phlegm-eliminating therapy on the NF-κB signaling pathway in a rat model of atherosclerosis (AS) and the mechanism of action of Huxinkang tablets in the treatment of AS.Methods：A rat model of AS was established by high-dose vitamin D3 injection and high-fat diet.The rats were randomly divided into high-，middle-，and low-dose Huxinkang tablet groups (at doses of 1.64，0.82，and 0.41g/kg，respectively)，positive control group (atorvastatin calcium tablets at a dose of 2 mg/kg)，model group (distilled water，10 ml/kg)，and blank group.The rats were sacrificed after 12 weeks of treatment.Double-antibody sandwich ELISA was used to measure the serum levels of the inflammatory factors interleukin-1β (IL-1β)，interleukin-6 (IL-6)，and tumor necrosis factor-α (TNF-α).Arterial tissue was collected，and Western blot was used to measure the protein expression of the NF-κB signaling pathway and an image analysis system was used to evaluate gray value.The protein expression of NF-κB in tissue was expressed as the ratio of interest protein to β-acting protein for internal control.Results：Compared with the model group，the middle- and high-dose Huxinkang tablet groups and the positive control group had significant reductions in the serum levels of IL-1β，IL-6，and TNF-α and the protein expression of the NF-κB signaling pathway (P<0.01).Conclusion：Qi-tonifying，blood-activating，and phlegm-eliminating therapy can downregulate the protein expression of the NF-κB signaling pathway，block the activation of this signaling pathway，inhibit the release of the inflammatory factors IL-1β，IL-6，and TNF-α，and thus exert a therapeutic effect on AS. |
| Key words: atherosclerosis rat Qi-tonifying,blood-activating,and phlegm-eliminating therapy NF-κB signaling pathway experimental study |
