| 摘要: |
| 目的：探讨参附注射液建立自发性高血压大鼠（SHR）模型的可能性。方法：将30只3月龄SPF级健康雄性自发性高血压大鼠称重，随机分为SHR组，附子汤组，参附注射液低、中、高剂量组，每组6只。各组采用对应药物灌胃进行模型制备，另取同品系同周龄雄性魏-凯二氏大鼠（WKY）6只作为正常对照组（WKY组）。观察参附注射液对SHR大鼠24h饮水量、易激惹程度、痛阈、睑结膜充血及收缩压的影响。结果：附子汤组及参附注射液中、高剂量组造模后均出现了易激惹程度增高、睑结膜充血程度增高、饮水量增多、痛阈降低、收缩压升高等肝阳上亢的表现，与WKY组及SHR组比较，差异有统计学意义（P<0.05)，表明造模成功。结论：参附注射液可以制备新型高血压肝阳上亢证模型，但是具体造模机制以及造模药物用量需待进一步探讨确定。 |
| 关键词: 原发性高血压 肝阳上亢证 动物模型 参附注射液 |
| DOI： |
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| A study of Shenfu injection in establishing a rat model of spontaneous hypertension with syndrome of upper hyperactivity of liver Yang |
| ZHOU Meng，,YANG Wenli，,LIU Xiaoqing |
| (Hunan University of Chinese Medicine，Changsha 410208，Hunan，China;The Affiliated Hospital of Hunan Academy of Chinese Medicine，Changsha 410006，Hunan，China) |
| Abstract: |
| Objective：To investigate the possibility of Shenfu injection in establishing a spontaneously hypertensive rat（SHR) model.Methods：A total of 30 specific pathogen-free healthy male SHRs aged 3 months were selected，weighed，and randomly divided into SHR group，Fuzi decoction group，and low-，middle-，and high-dose Shenfu injection groups，with 6 rats in each group.Each group was given the corresponding drug by gavage.A total of 6 make WKY rats with the same age were selected as normal controls（WKY group).The influence of Shenfu injection on 24-hour water intake，degree of irritability，pain threshold，conjunctival congestion，and systolic pressure was observed.Results：After modeling，the Fuzi decoction group and the middle- and high-dose Shenfu injection groups had significant increases in irritability，conjunctival congestion，water intake，and systolic pressure and a significant reduction in pain threshold，which were the manifestations of upper hyperactivity of liver Yang，and there were significant differences between the WKY group and the SHR group（P<0.05)，suggesting that a model was successfully established.Conclusion：Shenfu infection can be used to establish a model of hypertension with upper hyperactivity of liver Yang，but further studies are needed to clarify detailed mechanism and the dose of drug for modeling. |
| Key words: primary hypertension syndrome of upper hyperactivity of liver Yang animal model Shenfu injection |
