| 摘要: |
| 目的：探讨解毒化瘀颗粒对D-半乳糖胺/脂多糖诱导急性肝衰竭大鼠的保护作用及机制。方法：将30只SPF级雄性wistar大鼠随机分为治疗组、模型组、空白组，每组各10只。治疗组按10ml/kg体质量予解毒化瘀颗粒灌胃给药，每天2次，共7d；空白组及模型组给予等体积0.9%氯化钠注射液灌胃。于给药第6天，模型组与治疗组大鼠予腹腔注射D-半乳糖胺（600mg/kg）和脂多糖（20μg/kg）进行造模。24h后进行静脉取血，采用全自动生化仪检测血清中总胆红素（TBIL）、谷丙转氨酶（ALT）及谷草转氨酶（AST）水平，血栓止血分析仪检测血清中凝血酶原时间（PT）、血浆纤维蛋白（FIB）、活化部分凝血活酶时间（APTT）及凝血酶时间（TT）。处死大鼠取肝组织制备肝组织切片，光镜下观察肝组织结构。结果：与模型组比较，治疗组血清中的ALT、AST、TBIL水平明显降低，PT、TT时间缩短，FIB水平升高，2组ALT、TBIL、FIB水平及PT时间差异均具有统计学意义（P<0.05）。模型组大鼠肝细胞正常结构被破坏程度显著高于治疗组大鼠。结论：解毒化瘀颗粒可以通过改善肝功能、纠正凝血功能异常及减轻病理损伤而保护急性肝衰竭模型大鼠，其作用机制可能为通过CD4 T下调OX40表达，抑制CD4 T活化及向Th1细胞分化，从而延缓急性肝衰竭的进一步恶化。 |
| 关键词: 急性肝衰竭 大鼠 解毒化瘀颗粒 D-半乳糖胺/脂多糖 实验研究 |
| DOI： |
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| Protective effect of detoxicating and stasis-resolving granules against D-galactosamine/lipopolysaccharide-induced acute liver failure in rats and its mechanism of action |
| QIN Yanxin，,MA Yuzhen，,JIANG Qin |
| (Guangxi University of Chinese Medicine，Nanning 530001，Guangxi，China) |
| Abstract: |
| Objective：To investigate the protective effect of detoxicating and stasis-resolving granules against D-galactosamine/lipopolysaccharide-induced acute liver failure in rats and its mechanism of action.Methods：A total of 30 specific pathogen-free male Wistar rats were randomly divided into treatment group，model group，and blank group，with 10 rats in each group.The treatment group was intragastrically given detoxicating and stasis-resolving granules by 10ml/kg body weight，twice a day，for 7 days；the blank group and model group were intragastrically given an equal volume of 0.9% sodium chloride injection.D-galactosamine（600mg/kg) and lipopolysaccharide（20μg/kg) were intraperitoneally administered to the model group and treatment group for modeling on the 6th day of administration.Intravenous blood samples were collected at 24 hours after modeling，and tested by an automatic biochemical analyzer for serum levels of total bilirubin（TBIL)，alanine aminotransferase（ALT)，and aspartate aminotransferase（AST)；the samples were also tested by a hemostasis analyzer for prothrombin time（PT)，plasma fibrin（FIB)，activated partial thromboplastin time（APTT)，and thrombin time（TT).The rats were then sacrificed to collect their liver tissue which was prepared into sections；the liver tissue structure was observed under an optical microscope.Results：Compared with the model group，the treatment group had lower serum levels of ALT，AST，and TBIL，shorter PT and TT，and elevated FIB level；there were significant differences between the two groups in the levels of ALT，TBIL，and FIB，and PT（P<0.05).The degree of damage of normal structure of liver cells was significantly higher for rats in the model group than in the treatment group.Conclusion：Detoxicating and stasis-resolving granules may exert a protective effect on the rat model of acute liver failure by improving liver function，correcting coagulation abnormality，and alleviating pathological damage；the mechanism of the above process may involve down-regulation of OX40 expression by CD4 T cells，inhibition of activation of CD4 T cells and their differentiation into Th1 cells，thereby delaying further deterioration of acute liver failure. |
| Key words: acute liver failure rat detoxicating and stasis-resolving granule D-galactosamine/lipopolysaccharide experimental study |
