| 摘要: |
| 目的：探讨采用冠状动脉结扎法建立大鼠心肌纤维化（Myocardial Fibrosis，MF）模型的方法，以期为临床防治MF提供实验基础。方法：术前进行包括对实验动物、药物、仪器和器械的准备；手术操作过程按照麻醉、消毒、连接呼吸机、心电图监测、开胸、结扎、关胸、脱机、术后处理的步骤；手术过程要求无菌操作，保护实验动物肺脏，观察其呼吸及恢复自主呼吸时间，注意结扎手法，预防感染。造模是否成功可通过HE染色观察心脏组织病理学变化、Masson染色显微镜下进行左室心肌胶原容积分数检测、心脏组织HYP、TGF-β1含量检测来确定。结论：规范冠状动脉结扎法建立大鼠MF模型的步骤，完善每个细节，对提高实验大鼠存活率及造模成功率具有重要的意义。 |
| 关键词: 心肌纤维化 大鼠模型 冠状动脉结扎法 实验方法 |
| DOI： |
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| A discussion on the method for establishing a rat model of myocardial fibrosis using coronary artery ligation |
| LONG Yu，,DENG Juan-juan，,CAO Chu-heng |
| (Hunan University of Chinese Medicine，Changsha 410208，Hunan，China) |
| Abstract: |
| Objective：To investigate the method for establishing a rat model of myocardial fibrosis（MF）using coronary artery ligation，and to provide an experimental basis for clinical prevention and treatment of MF.Methods：Preoperative preparations included experimental animals，drugs，instruments，and devices.The surgery followed the steps of anesthesia，disinfection，connection with a respirator，ECG monitoring，thoracotomy，ligation，closure of the thoracic cavity，ventilator weaning，and postoperative treatment.The surgery required aseptic operation and protection of the lungs.Respiration and time to the recovery of spontaneous breathing were observed.Ligation was performed carefully，and related measures were adopted to prevent infection.In order to determine whether the model was established successfully，HE staining was used to observe cardiac histopathological changes，and Masson staining was used to measure collagen volume fraction in the left ventricle and the content of hydroxyproline（HYP）and transforming growth factor-β1（TGF-β1）in cardiac tissue under a microscope.Conclusion：Standardization of the steps for establishing a rat model of MF using coronary artery ligation and perfection of each detail help to improve the survival rate of experimental rats and the success rate of model establishment. |
| Key words: myocardial fibrosis rat model coronary artery ligation experimental method |
