| 摘要: |
| 目的：探讨咳喘宁防治呼吸道合胞病毒（RSV）诱发支气管哮喘的作用机制。方法：将60只2月龄豚鼠（雌雄各半）随机分为空白对照组，咳喘宁低、中、高剂量组，西药组，每组各10只（雌雄各半）。用RSV悬液滴鼻吸入后，卵清蛋白雾化豚鼠诱发哮喘模型，以地塞米松联合利巴韦林为阳性对照药，观察咳喘宁低、中、高剂量干预后糖原合成酶激酶3β（GSK-3β）、β-连环蛋白（β-catenin）的蛋白表达情况。结果：在RSV诱发的哮喘豚鼠肺组织中，与空白对照组比较，模型组肺组织中GSK-3β表达明显减少，β-catenin蛋白表达明显增加，差异具有统计学意义（P＜0.05）；与模型组比较，咳喘宁低、中、高剂量组及西药组豚鼠肺组织中GSK-3β表达增加，β-catenin表达减少，差异具有统计学意义（P＜P＜0.05）；西药组与咳喘宁低、中、高剂量组各项指标比较，差异均有统计学意义（P＜0.05）。结论：咳喘宁对RSV诱导的哮喘豚鼠具有抑制作用，其机制可能与WNT/β-catenin信号通路有关。 |
| 关键词: 哮喘 咳喘宁 GSK-3β β-catenin 实验研究 |
| DOI： |
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| Effect of Kechuanning on the expression of the GSK-3β and β-catenin signaling pathways in guinea pigs with asthma induced by respiratory syncytial virus |
| KONG Ding,DONG Xiaofei |
| (Hunan University of Chinese Medicine，Changsha 410208，Hunan，China;The First Affiliated Hospital of Hunan University of Chinese Medicine，Changsha 410007，Hunan，China) |
| Abstract: |
| Objective：To investigate the mechanism of action of Kechuanning in the prevention and treatment of bronchial asthma induced by respiratory syncytial virus (RSV).Methods：A total of 60 guinea pigs (30 male and 30 female guinea pigs)，aged 2 months，were randomly divided into blank control group，low-，middle-，and high-dose Kechuanning groups，and Western medicine group，with 10 guinea pigs (5 male and 5 female guinea pigs) in each group.Guinea pigs were given intranasally administered RSV suspension and ovalbumin aerosol to establish a model of asthma，and dexamethasone and ribavirin were used as positive control drugs to observe the protein expression of glycogen synthase kinase-3β (GSK-3β) and β-catenin after the intervention with low-，middle-，and high-dose Kechuanning.Results：In the lung tissue of guinea pigs with asthma induced by RSV，compared with the blank control group，the model group had a significant reduction in the expression of GSK-3β and a significant increase in the expression of β-catenin in lung tissue (P＜0.05).Compared with the model group，the low-，middle-，and high-dose Kechuanning groups and the Western medicine group had a significant increase in the expression of GSK-3β and a significant reduction in the expression of β-catenin in lung tissue (P＜0.05).There were significant differences in these indices between the Western medicine group and the low-，middle-，and high-dose Kechuanning groups (P＜0.05).Conclusion：Kechuanning has an inhibitory effect on guinea pigs with asthma induced by RSV，which may be associated with the WNT/β-catenin signaling pathway. |
| Key words: asthma Kechuanning glycogen synthase kinase-3β β-catenin experimental study |
