| 摘要: |
| 目的：通过脂多糖（LPS）干预单核细胞（THP-1）源性泡沫细胞制备高胆固醇模型，探讨右归丸含药血清对THP-1源性泡沫细胞胆固醇流出与炎症因子表达的影响。方法：利用LPS培养THP-1源性泡沫细胞诱导24h制备高胆固醇细胞模型，给予右归丸含药血清小、中、大剂量干预。通过比色法检测干预后的THP-1源性泡沫细胞内总胆固醇（TC）、游离胆固醇（FC）和胆固醇酯（CE）的含量；酶联免疫吸附法测定细胞内的细胞介素1β（IL-1β）、白细胞介素6（IL-6）、肿瘤坏死因子α（TNF-α）的含量；逆转录-聚合酶链反应法检测细胞NF-κB p65、SREBP1c、ABCA1mRNA的含量和蛋白表达。结果：10 ng/ml LPS培养THP-1源性泡沫细胞成功复制了高胆固醇细胞模型。右归丸大、中、小剂量组THP-1源性泡沫细胞内TC、FC、CE、IL-1β、IL-6、TNF-α、NF-κB p65、SREBP1c、ABCA1mRNA含量以及NF-κBp65、SREBP1c、ABCA1mRNA蛋白表达水平与正常组比较，差异均有统计学意义（P＜0.05或P<0.01）。结论：右归丸可能是通过抑制NF-κB p65和SREBP1c基因表达而调节血脂，同时通过抑制炎症因子的释放来预防动脉粥样硬化，为临床预防和治疗动脉粥样硬化提供了新的靶点。 |
| 关键词: THP-1源性泡沫细胞 右归丸 NF-κB SREBP-1c ABCA1 实验研究 |
| DOI： |
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| Effect of nuclear factor-kappa B activation on cholesterol efflux and expression of inflammatory factors in THP-1-derived foam cells and the intervention mechanism of Yougui pills |
| XU Anli,ZHAO Min,CHEN Huimin |
| (Hubei University of Chinese Medicine，Wuhan 430065，Hubei，China) |
| Abstract: |
| Objective：To establish a model of high cholesterol by lipopolysaccharide (LPS) intervention of THP-1-derived foam cells，and to investigate the effect of serum containing Yougui pills on cholesterol efflux and expression of inflammatory factors in THP-1-derived foam cells.Methods：THP-1-derived foam cells were cultured with LPS for 24 hours to establish a model of high cholesterol，and then the cells were given the intervention of low-，middle-，and high-dose serum containing Yougui pills.Colorimetry was used to measure the content of total cholesterol (TC)，free cholesterol (FC)，and cholesteryl ester (CE) in THP-1-derived foam cells after intervention；ELISA was used to measure the levels of interleukin-1β (IL-1β)，interleukin-6 (IL-6)，and tumor necrosis factor-α (TNF-α) in cells；RT-PCR was used to measure the mRNA expression of NF-κB p65，SREBP1c，and ABCA1 in cells.Results：The model of high cholesterol was successfully established by the intervention of THP-1-derived foam cells with 10 ng/ml LPS.There were significant differences in TC，FC，CE，IL-1β，IL-6，TNF-α，and mRNA and protein expression of NF-κB p65，SREBP1c，and ABCA1 between the normal group and the low-，middle-，and high-dose serum containing Yougui pills groups (P＜0.05 or P＜0.01).Conclusion：Yougui pills regulate blood lipids by inhibiting the mRNA expression of NF-κB p65 and SREBP1c and prevent atherosclerosis by inhibiting the release of inflammatory factors，which provides new targets for the prevention and treatment of atherosclerosis in clinical practice. |
| Key words: THP-1-derived foam cells Yougui pills NF-κB SREBP-1c ABCA1 experimental study |
