| 摘要: |
| 目的：观察宣痹通络膏对急性痛风性关节炎（AGA）模型大鼠髓样分化因子88（MyD88）表达的影响。方法：将60只雄性Wistar大鼠随机分为6组：宣痹通络膏低、中、高剂量组（简称为低、中、高剂量组），秋水仙碱组，模型组，空白组，每组各10只。于大鼠踝关节局部注射尿酸钠晶体混悬液制备AGA模型，造模后当天开始进行药物干预，连续3 d。观察各组大鼠不同时间点的关节肿胀度，血清及滑膜组织中MyD88蛋白表达水平，踝关节滑膜组织病理形态学变化。结果：关节肿胀度比较，24 h时，模型组较空白组上升；48h时，高剂量组、秋水仙碱组较模型组降低；72 h时，各给药组均较模型组降低，差异均有统计学意义（P<0.05）。与空白组比较，模型组大鼠血清、滑膜组织MyD88含量显著升高（P<0.01）；与模型组比较，各给药组血清MyD88含量以及高、中剂量组和秋水仙碱组滑膜组织MyD88蛋白含量均降低，差异均有统计学意义（P<0.05或P<0.01）。与模型组相比，各药物组的踝关节滑膜组织病理形态得到改善。结论：宣痹通络膏可能通过下调MyD88蛋白表达，从而改善AGA模型大鼠的踝关节滑膜损伤。 |
| 关键词: 急性痛风性关节炎 宣痹通络膏 MyD88 实验研究 |
| DOI： |
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| Effect of Xuanbi Tongluo ointment on the expression of myeloid differentiation factor 88 and the histomorphology of ankle joint synovial membrane in rats with acute gouty arthritis |
| WU Ruizhe,WU Jie,ZHANG Desheng |
| (The Second Affiliated Hospital of Hunan University of Chinese Medicine，Changsha 410005，Hunan，China) |
| Abstract: |
| Objective：To investigate the effect of Xuanbi Tongluo ointment on the expression of myeloid differentiation factor 88 （MyD88）and the histomorphology of ankle joint synovial membrane in rats with acute gouty arthritis （AGA）.Methods：A total of 60 male Wistar rats were randomly divided into low-，middle-，and high-dose Xuanbi Tongluo ointment groups （low-，middle-，and high-dose groups），colchicine group，model group，and blank group，with 10 rats in each group.The rats were given local injection of sodium urate crystal suspension to prepare a model of AGA，and drug intervention was given since the day of modeling for 3 consecutive days.The rats were observed in terms of joint swelling，protein expression level of MyD88 in serum and synovial membrane，and histopathological changes of ankle joint synovial membrane at different time points.Results：As for the comparison of joint swelling，the model group had a significant increase compared with the blank group at 24 hours；the high-dose group and the colchicine group had a significant reduction compared with the model group at 72 hours，and each administration group had a significant reduction compared with the model group （P<0.05）.Compared with the blank group，the model group had significant increases in the content of MyD88 in serum and synovial membrane （P<0.01）；compared with the model group，each administration group had a significant reduction in the content of MyD88 in serum，and the high-and middle-dose groups and the colchicine group had a significant reduction in the content of MyD88 in synovial membrane （P<0.05 or P<0.01）.Compared with the model group，each administration group had improvement in the histopathology of ankle joint synovial membrane.Conclusion：Xuanbi Tongluo ointment can improve the injury of ankle joint synovial membrane in a rat model of AGA possibly by downregulating the protein expression of MyD88. |
| Key words: acute gouty arthritis Xuanbi Tongluo ointment myeloid differentiation factor 88 experimental study |
