摘要: |
目的:观察茵陈四苓颗粒联合骨髓间充质干细胞(BMSCs)对急性肝衰竭大鼠线粒体膜电位的调节作用。方法:将60只雄性SPF级SD大鼠随机分成A组(正常组)、B组(茵陈四苓颗粒联合骨髓间充质干细胞组)、C组(茵陈四苓颗粒组)、D组(模型组)、E组(复方甘草酸苷组)、F组(骨髓间充质干细胞组),每组各10只。采用硫代乙酰胺腹腔注射的方法制备急性肝衰竭大鼠模型,进行BMSCs的分离纯化、培养及鉴定,观察大鼠的一般情况与存活情况,血清天冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、总胆红素(TBil)的含量,肝脏组织病理情况、肝线粒体的形态与结构、肝线粒体膜电位。结果:大鼠血清ALT、AST、TBil水平比较,D组较A组明显升高(P<0.05),B、C、E、F组较D组降低,其中B组降低更显著,差异均有统计学意义(P<0.05),E、F组组间差异无统计学意义(P>0.05)。B、C、E、F组肝脏组织病理、线粒体形态与结构的变化情况较D组好转。D组Q1-UR/Q1-LR阳性百分比比值显著升高,与A组比较,差异有统计学意义(P<0.05);B、C组与D组比较,差异有统计学意义(P<0.05);各干预组肝线粒体膜电位稳定,E组
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关键词: 急性肝衰竭 茵陈四苓颗粒 骨髓间充质干细胞 肝线粒体 膜电位 实验研究 |
DOI: |
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Regulatory effect of Yinchen Siling granules combined with bone marrow mesenchymal stem cells on mitochondrial membrane potential in rats with acute liver failure |
HU Yu,HU Yujie,ZHONG |
(Hospital T.C.M Affiliated to Southwest Medical University,Luzhou 646000,Sichuan,China) |
Abstract: |
Objective:To investigate the regulatory effect of Yinchen Siling granules combined with bone marrow mesenchymal stem cells (BMSCs) on mitochondrial membrane potential in rats with acute liver failure.Methods:A total of 60 specific pathogen-free male Sprague-Dawley rats were randomly divided into group A (normal group),group B (Yinchen Siling granules+BMSCs group),group C (Yinchen Siling granules group),group D (model group),group E (compound glycyrrhizin group),and group F (BMSCs group),with 10 rats in each group.Intraperitoneal injection of thioacetamide was performed to establish a rat model of acute liver failure,and BMSCs were isolated,purified,culture,and identified.The rats were observed in terms of general status,survival,serum content of aspartate aminotransferase (AST)/alanine aminotransferase (ALT)/total bilirubin (TBil),liver histopathology,liver mitochondrial morphology and structure,and liver mitochondrial membrane potential.Results:Compared with group A,group D had significantly higher serum levels of ALT,AST,and TBil (P<0.05);compared with group D,groups B,C,E,and F had significantly lower serum levels of ALT,AST,and TBil,and group B had significantly greater reductions than the other groups (P<0.05);there were no significant differences in these indices between group E and group F (P>0.05).Compared with group D,groups B,C,E,and F had significant improvements in liver histopathology and liver mitochondrial morphology and structure.Compared with group A,group D had a significant increase in the percentage of Q1-UR/Q1-LR-positive cells (P<0.05),and there was also a significant difference between group B/C and group D (P<0.05).Each intervention group had a stable liver mitochondrial membrane potential,and group B had the highest potential,followed by groups C,F,and E (P<0.05).Conclusion:Yinchen Siling granules combined with BMSCs exert a regulatory effect on mitochondrial membrane potential in rats with acute liver failure and can improve acute liver failure and protect the liver by inhibiting mitochondrial apoptosis. |
Key words: acute liver failure Yinchen Siling granules bone marrow mesenchymal stem cell liver mitochondria membrane potential experimental study |