| 摘要: |
| 目的：观察关节软坚止痛片对兔膝骨关节炎（KOA）模型血清及关节液中白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）的影响。方法：将40只新西兰兔随机分为5组：空白组、模型组、玻璃酸钠组（对照组）及关节软坚止痛片低、高剂量组（简称为低、高剂量组），每组各8只。采用屈曲位石膏铁丝固定法建立兔KOA模型后进行药物干预。观察比较各组肉眼下股骨内外髁组织，血清及关节液中IL-6、TNF-α含量，光镜下软骨组织形态学及Mankin's评分。结果：各组血清及关节液中IL-6、TNF-α水平及Mankin's评分比较，模型组均高于空白组、对照组及低、高剂量组（P<0.05），对照组均高于低、高剂量组（P<0.05），低、高剂量组比较，差异无统计学意义（P>0.05）。肉眼下股骨内外髁组织观察及光镜下软骨组织形态学观察结果显示：对照组及高、低剂量组均优于模型组，高、低剂量组均优于对照组。结论：关节软坚止痛片可治疗兔膝骨关节炎，其疗效优于玻璃酸钠注射液治疗，其作用机制可能与抑制IL-6、TNF-α等炎性因子表达有关。 |
| 关键词: 膝骨关节炎 关节软坚止痛片 IL-6 TNF-α 实验研究 |
| DOI： |
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| Effect of Guanjie Ruanjian Zhitong tablets on interleukin-6 and tumor necrosis factor-α in synovial fluid of rabbits with knee osteoarthritis |
| LIU Tingguo,MAO Ziqing,XIN Xiaochun |
| (Hunan University of Chinese Medicine，Changsha 410208，Hunan，China;Changsha Municipal Hospital of Traditional Chinese Medicine，Changsha 410002，Hunan，China) |
| Abstract: |
| Objective：To investigate the effect of Guanjie Ruanjian Zhitong tablets on interleukin-6 （IL-6） and tumor necrosis factor-α （TNF-α） in serum and synovial fluid of rabbits with knee osteoarthritis （KOA）.Methods：A total of 40 New Zealand rabbits were randomly divided into blank group，model group，sodium hyaluronate group （control group），and low-and high-dose Guanjie Ruanjian Zhitong tablets groups （low-and high-dose groups），with 8 rabbits in each group.The method of plaster-wire fixation at the flexion position was used to establish a rabbit model of KOA，and then drug intervention was given.The groups were compared in terms of themedial and lateral condyles of the femur under visual inspection，the content of IL-6 and TNF-α in serum and synovial fluid，cartilage histomorphologyunder a light microscope，and Mankins score.Results：The model group had significantly higher levels of IL-6 and TNF-α in serum and synovial fluid and Mankins score than the blank group，the control group，and the low-and high-dose groups （P<0.05），and these indices in the control group were significantly higher than those in the low-and high-dose groups （P<0.05），while there were no significant differences between the low-and high-dose groups （P>0.05）.Visual inspection of themedial and lateral condyles of the femur and cartilage histomorphologyunder a light microscope showed that the control group and the high-and low-dose groups had significantly better results than the model group，and the high-and low-dose groups had significantly better results than the control group.Conclusion：Guanjie Ruanjian Zhitong tablets can exert a therapeutic effect on KOA in rabbits and have a better effect than sodium hyaluronate injection，possibly by inhibiting the expression of the inflammatory factors such as IL-6 and TNF-α. |
| Key words: knee osteoarthritis Guanjie Ruanjian Zhitong tablets interleukin-6 tumor necrosis factor-α experimental study |
