| 摘要: |
| 目的：研究不同剂量益母缩宫颗粒对药物不全流产大鼠子宫内膜修复的影响。方法：选择雌性大鼠60只，雄性大鼠25只，随机挑选10只未合笼雌性大鼠为空白组，剩余大鼠按雌雄2∶1合笼，合笼成功后，予以米非司酮（8.3 mg/kg）+米索前列醇（100 μg/kg）制备药物不全流产大鼠模型。将造模成功后的雌性大鼠随机分为模型组，益母缩宫颗粒低、中、高剂量组，缩宫素组，予以相应的实验药物与对照药物进行干预。采用ELISA法检测血清纤维连接蛋白（FN）、层粘连蛋白（LN）、Ⅳ型胶原蛋白（ColIV）的水平，采用免疫组化法检测子宫组织中基质金属蛋白酶（MMP-9）、金属蛋白酶组织抑制因子（TIMP-1）的平均光密度（OD）值。结果：益母缩宫颗粒中、高剂量组及缩宫素组血清中FN、LN、ColIV水平及子宫组织中MMP-9、TIMP-1的OD值与模型组比较，差异均有统计学意义（P＜0.05或P＜0.01）。结论：益母缩宫颗粒能通过干预子宫内膜细胞外基质的降解与修复，减少子宫异常出血。 |
| 关键词: 药物不全流产 益母缩宫颗粒 子宫内膜修复 大鼠 实验研究 |
| DOI： |
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| Effect of Yimu Suogong granules on endometrial repair in rats with incomplete medical abortion |
| HUANG Zhengying,MAO Hui,LI Pinying |
| (The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University，Luzhou 646000，Sichuan，China) |
| Abstract: |
| Objective：To investigate the effect of different doses of Yimu Suogong granules on endometrial repair in rats with incomplete medical abortion.Methods：A total of 60 female rats and 25 male rats were selected，among which 10 uncaged female rats were randomly selected as blank group，and the remaining rats were caged with a female/male ratio of 2∶1.Then mifepristone (8.3 mg/kg) and misoprostol (100 μg/kg) were given to establish a rat model of incomplete medical abortion.After successful modeling，the female rats were randomly divided into model group，low-，middle-，and high-dose Yimu Suogong granules groups，and oxytocin group and were given intervention with corresponding experimental drugs and control drugs.ELISA was used to measure the serum levels of fibronectin (FN)，laminin (LN)，and type IV collagen (Col-IV)，and immunohistochemistry was used to measure the mean optical density (OD) of matrix metallopeptidase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in uterine tissue.Results：There were significant differences in the serum levels of FN，LN，and Col-IV and the OD values of MMP-9 and TIMP-1 in uterine tissue between the middle/high-dose Yimu Suogong granules groups and the model group，as well as between the oxytocin group and the model group (P＜0.05 or P＜0.01).Conclusion：Yimu Suogong granules can reduce abnormal uterine bleeding by regulating the degradation and repair of extracellular matrix in endometrium. |
| Key words: incomplete medical abortion Yimu Suogong granules endometrial repair rat experimental study |
