| 摘要: |
| 目的：观察电针联合补肾活血方对多囊卵巢综合征(PCOS)模型大鼠的作用机制。方法：将280只大鼠随机分为正常组（A组）、模型组（B组）、克罗米芬组（C组）、克罗米芬+补佳乐组（D组）、克罗米芬+电针组（E组）、克罗米芬+补肾活血方组（F组）、克罗米芬+电针+补肾活血方组（G组）。B、C、D、E、F、G组大鼠予以皮下注射脱氢表雄酮（DHEA）20 d建立PCOS模型，造模成功后C、D、E、F、G组分别给予相应药物干预，A、B组不作任何处理，连续干预5 d。观察大鼠动情周期的改变情况以及血清性激素［促卵泡生成激素（FSH）、促黄体生成激素（LH）、睾酮（T）、LH/FSH］水平、孕鼠数、总着床率、平均着床胚泡数。结果：动情期角化上皮细胞明显增多,未角化上皮细胞明显减少（P＜0.05）。各项性激素指标、总着床率、平均着床胚泡数方面，G组与A组比较，差异无统计学意义（P>0.05）；B、C、D、E、F组与A组比较，差异均有统计学意义（P＜0.05）。总着床率、平均着床胚泡数方面，E、F、G组与B组比较，G组与C、D、E、F组比较，差异均有统计学意义（P＜0.05或P＜0.01）。结论：电针、补肾活血方联合克罗米芬能有效改善PCOS大鼠性激素水平，提高PCOS大鼠总着床率和平均着床胚泡数。 |
| 关键词: PCOS 电针 补肾活血方 克罗米芬 大鼠 实验研究 |
| DOI： |
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| Effect of electroacupuncture combined with kidney-tonifying and blood-activating prescription on sex hormone and pregnancy rate in a rat model of polycystic ovary syndrome |
| JIANG Duosheng,LI Dan,WU Song |
| (Hubei Provincial Maternal and Child Health Hospital，Wuhan 430070，Hubei，China;Hubei University of Chinese Medicine，Wuhan 430061，Hubei，China) |
| Abstract: |
| Objective：To investigate the mechanism of action of electroacupuncture combined with kidney-tonifying and blood-activating prescription on a rat model of polycystic ovary syndrome (PCOS).Methods：A total of 280 rats were randomly divided into normal group (group A)，model group (group B)，clomiphene group (group C)，clomiphene+Progynova group (group D)，clomiphene+electroacupuncture group (group E)，clomiphene+kidney-tonifying and blood-activating prescription group (group F)，and clomiphene+electroacupuncture+kidney-tonifying and blood-activating prescription (group G).The rats in groups B，C，D，E，F，and G were subcutaneously injected with dehydroepiandrosterone for 20 days to establish a model of PCOS，and after successful modeling，the rats in groups C，D，E，F，and G were given corresponding drug interventions for 5 consecutive days，while no treatment was performed for groups A and B.The rats were observed in terms of the change in estrous cycle，serum levels of sex hormones ［follicle-stimulating hormone (FSH)，luteinizing hormone (LH)，testosterone (T)，and LH/FSH］，number of pregnant rats，overall implantation rate，and mean number of implanted blastocysts.Results：There was a significant increase in keratinizing epithelial cells and a significant reduction in non-keratinizing epithelial cells in the estrous cycle (P＜0.05).There were no significant differences between groups G and A in sex hormones，overall implantation rate，and mean number of implanted blastocysts (P>0.05)，while there were significant differences in these indices between groups B/C/D/E/F and group A (P＜0.05).There were significant differences in overall implantation rate and mean number of implanted blastocysts between groups E/F/G and group B and between group G and groups C/D/E/F (P＜0.05 or P＜0.01).Conclusion：Electroacupuncture combined with kidney-tonifying and blood-activating prescription and clomiphene can effectively improve levels of sex hormones，overall implantation rate，and mean number of implanted blastocysts in rats with PCOS. |
| Key words: polycystic ovary syndrome electroacupuncture kidney-tonifying and blood-activating prescription clomiphene rat experimental study |
