| 摘要: |
| 目的：观察银杏提取物EGb761对大鼠视神经损伤后视网膜神经节细胞自噬活性及自噬流的影响，并探讨其作用机制。方法：将48只SD大鼠随机分为EGb761组和对照组，分别给予EGb761（150mg/kg·d）和0.9%氯化钠注射液灌胃，均每天1次，直到处死。分别于损伤后第7天、第14天取材，采用免疫组化检测2组视网膜神经节细胞自噬相关蛋白LC3B、自噬标记蛋白p62及自噬基因Beclin-1及mTOR通路蛋白p-mTOR、p-S6表达水平，腺病毒GFP-mRFP-LC3荧光瞬时转染技术检测自噬流表达。结果：与对照组相比，EGb761组LC3B表达水平升高，p62、p-mTOR和p-S6蛋白表达水平均下降（均P<0.05）；2组Beclin-1差异无统计学意义（P＞0.05）；2组GFP和mRFP荧光点数比较，EGb761组的红点多于对照组，黄点少于对照组（均P<0.05）。结论：EGb761能够促进大鼠视神经损伤后视网膜神经节细胞自噬流，其机制可能与促进mTOR信号通路中的相关蛋白表达有关。 |
| 关键词: EGb761 视网膜神经节细胞 自噬 实验研究 |
| DOI： |
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| Effect of EGb761 on the autophagy of ganglion cells after optic nerve damage |
| QIAO Feng,CHENG,Yun-cui |
| (Hengyang Central Hospital，Hengyang 421001，Hunan，China) |
| Abstract: |
| Objective：To investigate the effect of EGb761，a gingko extract，on the autophagy activity and autophagy flux of retinal ganglion cells after optic nerve damage in rats and its mechanism of action.Methods：A total of 48 Sprague-Dawley rats were randomly divided into EGb761 group and control group and were treated with EGb761（150 mg/kg/day) or 0.9% sodium chloride injection by gavage once a day until being sacrificed.Samples were collected at 7 and 14 days after damage；immunohistochemistry was used to measure the expression of autophagy-related protein microtubule-associated protein light chain 3B（LC3B)，autophagy marker protein p62，autophagic gene Beclin-1，and mTOR pathway proteins p-mTOR and p-S6，and adenovirus GFP-mRFP-LC3 transient transfection was used to measure the expression of autophagy flux.Results：Compared with the control group，the EGb761 group had a significant increase in the expression of LC3B and significant reductions in the expression of p62，p-mTOR，and p-S6（all P<0.05).There was no significant difference in Beclin-1 between the two groups（P>0.05).As for the comparison of fluorescence spots of GFP and mRFP，the EGb761 group had more red spots and fewer yellow spots than the control group（both P<0.05).Conclusion：EGb761 can promote autophagy flux of retinal ganglion cells after optic nerve damage in rats，possibly by promoting the expression of proteins involved in the mTOR signaling pathway. |
| Key words: EGb761 retinal ganglion cell autophagy experimental study |
