| 摘要: |
| 目的:研究卫矛醇对C6胶质瘤细胞株的体外增殖及凋亡的影响。方法：将C6大鼠胶质瘤细胞随机分为对照组、卫矛醇干预组、卫矛醇+雷帕霉素组、卫矛醇+3-MA组，各组予不同药物孵育，采用MTT法检测卫矛醇对C6细胞的体外增殖抑制率，筛选出抑制率相对较高且生长状态良好的细胞；应用Hoechest 33342染色透射电镜观察细胞凋亡亚显微结构的改变，采用免疫荧光和Western Blot技术检测与凋亡和自噬相关蛋白的表达水平。结果:卫矛醇对C6胶质瘤细胞增殖有明显的抑制作用，并呈现出良好的浓度-效应相关性，与对照组比较，随着浓度的增加，细胞的增殖抑制率上升(P＜0.01)；Hoechst染色结果显示，与对照组相比，共同孵育卫矛醇24 h后的C6细胞出现明显的核固缩现象，证明有大量的细胞出现凋亡；Western Blot结果显示，与对照组相比，促凋亡基因Bax表达上调，抗凋亡基因Bcl-2表达下调。结论:卫矛醇能够抑制C6细胞的增殖，并诱导其凋亡，其作用机制可能与上调Bax、下调Bcl-2有关。 |
| 关键词: 卫矛醇 C6胶质瘤细胞 抗癌活性 作用机制 |
| DOI： |
|
|
| In vitro antitumor activity of dulcitol on C6 glioma cells and related mechanism |
| ZHANG Yuling,ZHANG Tao,CHEN Baogui |
| (Beichen Hospital of Traditional Chinese Medicine，Tianjin 300499，China;School of Life Sciences，Nankai University，Tianjin 300071，China;Wuqing Hospital of Traditional Chinese Medicine，Tianjin 301799，China) |
| Abstract: |
| Objective:To investigate the effect of dulcitol on the in vitro proliferation and apoptosis of C6 glioma cell line.Methods：Rat C6 glioma cells were randomly divided into control group，dulcitol intervention group，dulcitol+rapamycin group，and dulcitol+3-MA group，and these groups were incubated with different drugs.MTT assay was used to measure the inhibition rate of dulcitol on the in vitro proliferation of C6 cells，and the cells with a relatively high inhibition rate and good growth status were obtained；Hoechest33342 staining and transmission electron microscopy were used to observe the change in cell apoptosis and submicroscopic structure；immunofluorescence assay and Western blot were used to measure the expression levels of apoptosis- and autophagy-related proteins.Results：Dulcitol showed a marked inhibitory effect on the proliferation of C6 glioma cells，with a good concentration-effect relationship，and compared with the control group，the inhibition rate of cell proliferation increased with the increase in concentration (P<0.01).Hoechst staining showed that compared with the control group，C6 cells incubated with dulcitol for 24 hours had marked karyopyknosis，suggesting the apoptosis of a large number of cells.Western blot showed that compared with the control group，there was an increase in the expression of the pro-apoptotic gene Bax and a reduction in the expression of the anti-apoptotic gene Bcl-2.Conclusion：Dulcitol can inhibit the proliferation of C6 cells and induce the apoptosis of C6 cells，possibly by upregulating Bax and downregulating Bcl-2. |
| Key words: dulcitol C6 glioma cells antitumor activity mechanism of action |
