| 摘要: |
| 目的：证实麻杏石甘汤对脂多糖（LPS）诱导的发热模型大鼠的解热作用，探究其解热机制及其对大鼠脑组织硫化氢（H22S）/胱硫醚-β-合成酶（CBS）体系的影响。方法：将50只无特定病原体（SPF）SD大鼠随机分为空白组、对照组（蒸馏水）、阿司匹林组（0.125 g/kg阿司匹林肠溶片）、麻杏石甘汤低剂量组（3.125 g/kg麻杏石甘汤)、麻杏石甘汤高剂量组（6.250 g/kg麻杏石甘汤），每组各10只。连续灌胃5 d，末次灌胃1 h后，各给药组予以腹腔注射20 μg/kg LPS溶液诱导大鼠出现发热模型，空白组予以等剂量0.9%氯化钠注射液。记录造模后5 h内大鼠肛温的变化情况。末次体温测量后，腹腔注射3%戊巴比妥钠麻醉大鼠，经腹主动脉收集血液，通过酶联免疫法（ELISA）检测血清白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）、细胞干扰素-α（IFN-α）的水平；取大鼠脑组织，运用实时荧光定量聚合酶链式反应（RT-qPCR）测定脑组织中胱硫醚-β-合成酶（CBS）、硫化氢（H22S） mRNA的表达。结果：与对照组比较，麻杏石甘汤低、高剂量组具有良好的解热效果，且麻杏石甘汤低、高剂量组均能抑制发热大鼠血清中IL-1β、TNF-α的表达，麻杏石甘汤高剂量组能抑制发热大鼠血清IFN-α的表达，差异均有统计学意义（P<0.05）。麻杏石甘汤低、高剂量组CBS、H22S mRNA的含量较对照组显著降低，差异有统计学意义（P<0.01）。结论：麻杏石甘汤具有良好的解热作用，其机制可能与减少血清中炎症因子的释放有关；同时麻杏石甘汤能降低发热模型大鼠脑组织中CBS、H22S mRNA的表达，并可能发挥了其对脑组织的保护作用。 |
| 关键词: 麻杏石甘汤 发热 炎性因子 硫化氢 胱硫醚-β-合成酶 |
| DOI： |
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| Antipyretic effect of Maxing Shigan decoction in a rat model of pyrexia and its effect on the hydrogen sulfide/cystathionine-β-synthase system |
| LONG Yuan,HE Siyu,ZHU Qinquan |
| (The First Clinical Traditional Chinese Medicine College of Hunan University of Chinese Medicine，Changsha 410007，Hunan，China;The First Affiliated Hospital of Hunan University of Chinese Medicine，Changsha 410007，Hunan，China) |
| Abstract: |
| Objective: To investigate the antipyretic effect of Maxing Shigan decoction in a rat model of pyrexia induced by lipopolysaccharide (LPS) and related mechanisms，as well as its effect on the hydrogen sulfide (H22S)/cystathionine-β-synthase (CBS) system in rat brain tissue.Methods: A total of 50 specific pathogen-free Sprague-Dawley rats were randomly divided into blank group，control group (distilled water)，aspirin group (0.125 g/kg enteric-coated aspirin tablets)，low-dose Maxing Shigan decoction group (3.125 g/kg Maxing Shigan decction)，and high-dose Maxing Shigan decoction group (6.250 g/kg Maxing Shigan decoction)，with 10 rats in each group.The drugs were given by gavage for 5 days，and at 1 hour after the last administration by gavage，the rats in each administration group were given intraperitoneal injection of 20 μg/kg LPS solution to induce pyrexia in rats，while those in the blank group were given an equal dose of 0.9% sodium chloride injection.The change in rectal temperature was recorded within 5 hours after modeling.After measurement of body temperature for the last time，3% pentobarbital sodium was intraperitoneally injected for anesthesia of rats，and blood was collected via the abdominal aorta.ELISA was used to measure the serum levels of interleukin-1β (IL-1β)，tumor necrosis factor-α (TNF-α)，and interferon-α (IFN-α).Rat brain tissue was collected，and RT-qPCR was used to measure the mRNA expression levels of CBS and H22S in brain tissue.Results: Compared with the control group，the low- and high-dose Maxing Shigan decoction groups showed a good antipyretic effect.Both low- and high-dose Maxing Shigan decoction could inhibit the expression of IL-1β and TNF-α in serum，and high-dose Maxing Shigan decoction could significantly inhibit the expression of IFN-α in serum (P<0.05).Compared with the control group，the low- and high-dose Maxing Shigan decoction groups showed significant reductions in the mRNA expression levels of CBS and H22S (P<0.01).Conclusion: Maxing Shigan decoction has a good antipyretic effect，possibly by reducing the release of inflammatory factors in serum; meanwhile，Maxing Shigan decoction reduces the mRNA expression levels of CBS and H22S in rat brain tissue and may exert a protective effect on brain tissue. |
| Key words: Maxing Shigan decoction pyrexia inflammatory factor hydrogen sulfide cystathionine-β-synthase |
