| 摘要: |
| 目的：通过评价羚珠散对C57BL/6幼鼠反复热惊厥的减轻作用，探讨其防治热惊厥的药效和可能作用机制。方法：将7 d龄C57BL/6幼鼠30只分为空白对照组、模型对照组和治疗组，每组各10只。采用热水浴法干预模型对照组与治疗组，生成惊厥模型，每次诱导前30 min治疗组灌胃给药羚珠散（1.5 g/kg），模型对照组灌胃同等体积0.9%氯化钠注射液。观察治疗组与模型对照组幼鼠的惊厥潜伏期和惊厥持续时间的差异，通过HE染色比较各组间幼鼠海马体病理组织学差异，通过Real-time PCR与IHC分析比较空白对照组、模型对照组及治疗组幼鼠海马体中Gabbr1和Kcnt1基因的表达差异。结果：经7次热水浴后，模型对照组幼鼠的惊厥潜伏时间为（32.80±3.27）s，治疗组幼鼠的惊厥潜伏时间为（50.90±6.88）s，治疗组的惊厥潜伏时间长于模型对照组（P＜0.05）；模型对照组幼鼠的惊厥持续时间为（104.40±14.37）s，治疗组幼鼠的惊厥持续时间为（45.40±7.10）s，治疗组的惊厥持续时间显著短于模型对照组（P＜0.01）；HE 染色显示热惊厥状态对模型对照组幼鼠海马神经元损伤明显，细胞肿胀、核皱缩，出现空泡现象，治疗组幼鼠海马神经元损伤减轻，细胞水肿及空泡等现象减轻；治疗组幼鼠中Gabbr1和Kcnt1的 mRNA表达水平高于模型对照组幼鼠（P＜0.05）；免疫组化检测幼鼠海马区中Gabbr1和Kcnt1的阳性细胞比例治疗组高于模型对照组（P＜0.01，P＜0.05）。结论：羚珠散具有显著防治幼鼠热惊厥发作的作用，其机制可能与显著提高Gabbr1与Kcnt1表达，增强GABA抑制神经兴奋作用，减轻海马区神经元损伤有关。 |
| 关键词: 惊厥 羚珠散 Gabbr1 Kcnt1 动物实验 幼鼠 |
| DOI： |
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| Role and mechanism of Lingzhu powder in prevention and treatment of recurrent febrile convulsion in young mice |
| WANG Haijie,WANG Chengji,LU Weisheng |
| (Shanghai Laboratory Animal Research Center，Shanghai 201203，China) |
| Abstract: |
| Objective：To investigate the effect and possible mechanism of action of Lingzhu powder in the prevention and treatment of recurrent febrile convulsion in young mice by evaluating whether Lingzhu powder can alleviate recurrent febrile convulsion in young C57BL/6 mice. Methods：A total of 30 young C57BL/6 mice，aged 7 days，were divided into blank control group，model control group，and treatment group，with 10 mice in each group. The mice in the model control group and the treatment group were treated with hot water bath to establish a model of convulsion; the mice in the treatment group were given Lingzhu powder (1.5 g/kg) by gavage at 30 minutes before induction，and those in the model control group were given an equal volume of 0.9% sodium chloride injection by gavage. The treatment group and the model control group were compared in terms of convulsion latency and convulsion duration; HE staining was used to compare the histopathology of the hippocampus between groups; real-time PCR and immunohistochemistry were used to compare the mRNA expression levels of Gabbr1 and Kcnt1 in the hippocampus between groups. Results：After 7 hot water baths，the treatment group had a significantly longer convulsion latency than the model control group (50.90±6.88 seconds vs 32.80±3.27 seconds，P＜0.05); the treatment group had a significantly shorter convulsion duration than the model control group (45.40±7.10 seconds vs 104.40±14.37 seconds，P＜0.01). HE staining showed that the state of febrile convulsion caused significant neuronal injury in the hippocampus of mice in the model control group，with cell swelling，nuclear shrinkage，and vacuolization，while the treatment group had alleviation of neuronal injury，cell swelling，and vacuolization. The treatment group had significantly higher mRNA expression levels of Gabbr1 and Kcnt1 in the hippocampus than the model control group (P＜0.05)，and immunohistochemistry showed that compared with the model control group，the treatment group had a significantly higher percentage of cells positive for Gabbr1 and Kcnt1 in the hippocampus (P＜0.01 and P＜0.05). Conclusion：Lingzhu powder has a marked effect in the prevention and treatment of febrile convulsion in young mice，possibly by significantly increasing the expression of Gabbr1 and Kcnt1，enhancing the effect of GABA in inhibiting nervous excitation，and alleviating neuronal injury in the hippocampus. |
| Key words: convulsion Lingzhu powder Gabbr1 Kcnt1 animal experiment young mice |
